Arylamine N-acetyltransferase 1 gene regulation by androgens requires a conserved heat shock element for heat shock factor-1

Butcher, Neville J. and Minchin, Rodney F. (2010) Arylamine N-acetyltransferase 1 gene regulation by androgens requires a conserved heat shock element for heat shock factor-1. Carcinogenesis, 31 5: 820-826.


Author Butcher, Neville J.
Minchin, Rodney F.
Title Arylamine N-acetyltransferase 1 gene regulation by androgens requires a conserved heat shock element for heat shock factor-1
Formatted title Arylamine N-acetyltransferase 1 gene regulation by androgens requires a conserved heat shock element for heat shock factor-1
Journal name Carcinogenesis  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2010-05
Sub-type Article
DOI 10.1093/carcin/bgq042
Volume number 31
Issue number 5
ISSN 1460-2180; 0143-3334
Start page 820
End page 826
Total pages 7
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2011
Language eng
Subject C1
920102 Cancer and Related Disorders
0601 Biochemistry and Cell Biology
Formatted abstract Human arylamine N-acetyltransferase 1 (NAT1) is a widely distributed protein that has been implicated in a number of different cancers including breast and prostate. Previously, NAT1 gene expression was shown to be androgen dependent, although the effect of androgens was not due to direct activation of the NAT1 promoter. Here, we show that heat shock factor (HSF)1 is induced by androgen in androgen receptor-positive prostate 22Rv1 cells. It also binds to a heat shock element (HSE) in the NAT1 promoter located 776 bp upstream of the transcriptional start site. Mutation of the HSE inhibited androgen responsiveness and prevented direct upregulation of the NAT1 promoter by HSF1. Although HSF2 also bound to the HSE, it did not increase promoter activity. HSF1 induced endogenous NAT1 activity in this cell line in the absence of androgen. This could be attenuated by pretreating cells with HSF1-directed small interfering RNA but not by a scrambled sequence. Our results show that HSF1 is an important transcription factor for induction of NAT1 in human cells and is required for androgen activation of the NAT1 promoter.
Copyright © 2011 Oxford University Press
Keyword Heterocyclic Amine Carcinogens
Prostate-cancer
Transcription Factor-1
N-Acetyltransferase-1
Expression
Binding
Cells
Promoter
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article
Collections: Official 2011 Collection
School of Biomedical Sciences Publications
 
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Created: Sun, 23 May 2010, 00:05:35 EST