Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue

Louis, Sharon A. and Reynolds, Brent A. (2005) Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue. Methods in Molecular Biology, 290 265-280. doi:10.1385/1-59259-838-2:265


Author Louis, Sharon A.
Reynolds, Brent A.
Title Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue
Journal name Methods in Molecular Biology   Check publisher's open access policy
ISSN 1064-3745
1940-6029
Publication date 2005
Sub-type Article (original research)
DOI 10.1385/1-59259-838-2:265
Volume 290
Start page 265
End page 280
Total pages 16
Editor Cheryl D. Helgason
Cindy L. Miller
Place of publication New York, NY, United States
Publisher Humana Press
Language eng
Subject 0601 Biochemistry and Cell Biology
Abstract Murine embryonic day 14 or E14 neural stem cells (NSCs), first isolated and characterized as a stem cell in culture, are a unique population of cells capable of self-renewal. In addition, they produce a large number of progeny capable of differentiating into the three primary phenotypes—neurons, astrocytes, and oligodendrocytes’found in the adult mammalian central nervous system (CNS). A defined serum-free medium supplemented with epidermal growth factor (EGF) is used to maintain the NSCs in an undifferentiated state in the form of clusters of cells, called neurospheres, for several culture passages. When EGF is removed and serum added to the medium, the intact or dissociated neurospheres differentiate into the three primary CNS phenotypes. This chapter outlines the simple NSC culture methodology and provides some of the more important details of the assay to achieve reproducible cultures.
Keyword Murine
Embryonic neural stem cells
Neurospheres
Differentiation
CNS
Culture
Stem cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes This is a chapter within a monagraph series in the book: Basic Cell Culture Protocols, Third Edition, chapter 18, ISBN 978-1-58829-284-1 (Print); 978-1-59259-838-0 (Online)

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Queensland Brain Institute Publications
 
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Created: Tue, 18 May 2010, 14:03:37 EST by Laura McTaggart on behalf of Queensland Brain Institute