Non-invasive methods of oestrus detection in captive southern hairy-nosed wombats (Lasiorhinus latifrons)

Hogan, L. A., Phillips, C. J. C., Lisle, A., Keeley, T., Horsup, A. B., Janssen, T. and Johnston, S. D. (2010) Non-invasive methods of oestrus detection in captive southern hairy-nosed wombats (Lasiorhinus latifrons). Animal Reproduction Science, 119 3-4: 293-304. doi:10.1016/j.anireprosci.2010.01.006


Author Hogan, L. A.
Phillips, C. J. C.
Lisle, A.
Keeley, T.
Horsup, A. B.
Janssen, T.
Johnston, S. D.
Title Non-invasive methods of oestrus detection in captive southern hairy-nosed wombats (Lasiorhinus latifrons)
Formatted title
Non-invasive methods of oestrus detection in captive southern hairy-nosed wombats (Lasiorhinus latifrons)
Journal name Animal Reproduction Science   Check publisher's open access policy
ISSN 0378-4320
Publication date 2010-06
Sub-type Article (original research)
DOI 10.1016/j.anireprosci.2010.01.006
Volume 119
Issue 3-4
Start page 293
End page 304
Total pages 12
Collection year 2011
Language eng
Formatted abstract
In order to develop a reliable method of oestrus detection in captive southern hairy-nosed
(SHN) wombats, the reproductive behaviour of four groups of adult animals (1♂:2♀) was
monitored using video surveillance and activity using movement-sensitive radio transmitters
for a period of 12 months. During this time faecal samples were collected every 3
days and subsequently analysed for progesterone and oestradiol-17β metabolites. In an
attempt to induce and characterise oestrus-specific behaviour, each female was administered
a subcutaneous injection of either 0.01 (n = 2), 0.1 (n = 4) or 0.2 mg/kg (n=2) of
oestradiol benzoate in one of two hormone trials. Remote video surveillance was an effective
tool for detecting the reproductive behaviour of the captive SHN wombat. Courtship
(n = 426) and mating (n = 46) was observed in five wombats and consisted of 13 distinctive
behaviours in six consecutive phases: (1) investigation, (2) attraction, (3) chase, (4)
restraint, (5) copulation and (6) recovery. Female sexual receptivity occurred at night and
lasted for approximately only 13-h. Faecal progesterone metabolite analysis proved to be a
reliable method for mapping oestrous cycle activity, but was not useful for the prediction
of oestrus. Six out of the eight female wombats displayed periods of elevated progesterone
secretion, corresponding to a mean (±SE) luteal phase of 20.9±1.1 days (n = 23). Oestrous
cycle length, defined as the interval between two successive luteal phases separated by a
follicular phase was 31.8±1.1 days (n = 12) and consisted of a follicular phase of 11.6±0.6
days (n = 12). Changes in the secretion of faecal oestradiol-17 metabolites provided little
instructive information on oestrous cycle activity and were not associated with oestrus.
Administration of oestradiol benzoate resulted in a spike of oestradiol-17β metabolites in
the faeces 3–4 days later, but was not dose dependent nor did it facilitate reproductive
behaviour in either sex. Activity was not linked to key events in the oestrous cycle and
appears not to be suitable as a method for detecting oestrus in the SHN wombat. We therefore
recommend the use of 24-h video surveillance as the most reliable method for oestrus
detection in captive SHN wombats.
© 2010 Elsevier B.V. All rights reserved.
Keyword Faecal steroids
Progesterone
Oestradiol benzoate
Reproductive behaviour
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 16 May 2010, 00:08:50 EST