Disparate infection patterns of Ceratomyxa shasta (Myxozoa) in rainbow trout (Oncorhynchus mykiss) and Chinook salmon (Oncorhynchus tshawytscha) correlate with internal transcribed spacer-1 sequence variation in the parasite

Atkinson, Stephen D. and Bartholomew, Jerri L. (2010) Disparate infection patterns of Ceratomyxa shasta (Myxozoa) in rainbow trout (Oncorhynchus mykiss) and Chinook salmon (Oncorhynchus tshawytscha) correlate with internal transcribed spacer-1 sequence variation in the parasite. International Journal For Parasitology, 40 5: 599-604. doi:10.1016/j.ijpara.2009.10.010


Author Atkinson, Stephen D.
Bartholomew, Jerri L.
Title Disparate infection patterns of Ceratomyxa shasta (Myxozoa) in rainbow trout (Oncorhynchus mykiss) and Chinook salmon (Oncorhynchus tshawytscha) correlate with internal transcribed spacer-1 sequence variation in the parasite
Formatted title
Disparate infection patterns of Ceratomyxa shasta (Myxozoa) in rainbow trout (Oncorhynchus mykiss) and Chinook salmon (Oncorhynchus tshawytscha) correlate with internal transcribed spacer-1 sequence variation in the parasite
Journal name International Journal For Parasitology   Check publisher's open access policy
ISSN 0020-7519
1879-0135
Publication date 2010-04
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.ijpara.2009.10.010
Volume 40
Issue 5
Start page 599
End page 604
Total pages 6
Place of publication Kidlington, Oxford., United Kingdom
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
Ceratomyxa shasta is a virulent myxosporean parasite of salmon and trout in the Pacific Northwest of
North America. The parasite is endemic in the Klamath River, Oregon/California, where a series of dams
prevent movement of fish hosts between the upper and lower parts of the basin. Ceratomyxa shasta exhibits
a range of infection patterns in different fish species above and below the dams. We hypothesised that
the variations in infection and disease are indicators that different strains of the parasite exist, each with
distinct host associations. Accordingly, we sought to identify strain-specific genetic markers in the
ssrRNA and internal transcribed spacer region 1 (ITS-1). We examined 46 C. shasta isolates from water
samples and two fish hosts, from June 2007 field exposures at upper and lower Klamath River sites with
similarly high parasite densities. We found 100% of non-native rainbow trout became infected and died at
both locations. In contrast, mortality in native Chinook salmon was <10% in the upper basin, compared
with up to 40% in the lower basin. Parasite ssrRNA sequences were identical from all fish. However,
ITS-1 sequences contained multiple polymorphic loci and a trinucleotide repeat (ATC)0–3 from which
we defined four genotypes: 0, I, II and III. Non-native rainbow trout at both sites were infected with genotype
II and with a low level of genotype III. Chinook salmon in the upper basin had genotypes II and III,
whereas in the lower basin genotype I predominated. Genotype I was not detected in water from the
upper basin, a finding consistent with the lack of anadromous Chinook salmon there. Genotype O was
only detected in water from the upper basin. Resolution of C. shasta into sympatric, host-specific genotypes
has implications for taxonomy, monitoring and management of this significant parasite.
2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
Keyword Ceratomyxa shasta
Myxozoa
Myxosporean
ITS-1
Parasite
Genotype
Klamath River
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 4 November 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 29 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 16 May 2010, 00:05:39 EST