Localisation of three host-protective oncospheral antigens of Taenia ovis

Jabbar, Abdul, Kyngdon, Craig T., Gauci, Charles G., Walduck, Anna K., McCowan, Christina, Jones, Malcolm K., Beveridge, Ian and Lightowlers, Marshall W. (2010) Localisation of three host-protective oncospheral antigens of Taenia ovis. International Journal For Parasitology, 40 5: 579-589. doi:10.1016/j.ijpara.2009.10.008


Author Jabbar, Abdul
Kyngdon, Craig T.
Gauci, Charles G.
Walduck, Anna K.
McCowan, Christina
Jones, Malcolm K.
Beveridge, Ian
Lightowlers, Marshall W.
Title Localisation of three host-protective oncospheral antigens of Taenia ovis
Formatted title
Localisation of three host-protective oncospheral antigens of Taenia ovis
Journal name International Journal For Parasitology   Check publisher's open access policy
ISSN 0020-7519
1879-0135
Publication date 2010-04
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.ijpara.2009.10.008
Volume 40
Issue 5
Start page 579
End page 589
Total pages 11
Place of publication London, England
Publisher Elsevier Science
Collection year 2011
Language eng
Abstract Immunohistochemistry, confocal immunofluorescence and immunogold labelling were used to determine the localisation of the host-protective antigens To16, To18 and To45W in Taenia ovis oncospheres. During maturation of the adult tapeworm the antigens were initially seen as diffuse staining in the developing oncospheres but in mature oncospheres four distinct cells stained positively for the antigens. Confocal fluorescence microscopy using different fluorophores revealed that each of the antigens co-localises within the same cells in the oncosphere. No surface localisation was seen in non-activated or recently activated parasites. Immunogold labelling of non-activated oncosphere sections viewed in transmission electron microscopy revealed labelling of bilateral cells, however the identities of these cells was unclear due to deficiencies in the current level of understanding of oncosphere ultrastructure. Localisation of all the antigens changed dramatically after oncospheres were activated in vitro with each of the antigens being dispersed more generally throughout the parasite parenchyma. During development of the parasites in in vitro culture, surface localisation of the proteins was seen in parasites after 3 or more days in culture. All three antigens were found to be completely absent in parasites by 15 days of culture. The location of the host-protective antigens suggests that initially the invading oncospheres are not susceptible to vaccine-induced antibody and complement mediated attack, but that as the parasites mature, the host-protective antigens come to be associated with the parasite’s surface, rendering them susceptible to immune attack.
Keyword Taenia ovis
Vaccine antigen
Oncosphere
Immunohistochemistry
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 31 October 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Veterinary Science Publications
 
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Created: Sun, 16 May 2010, 00:05:31 EST