Efficient siRNA delivery to mammalian cells using layered double hydroxide nanoparticles

Ladewig, Katharina, Niebert, Marcus, Xu, Zhi P., Gray, Peter P. and Lu, Gao Q. M. (2010) Efficient siRNA delivery to mammalian cells using layered double hydroxide nanoparticles. Biomaterials, 31 7: 1821-1829. doi:10.1016/j.biomaterials.2009.10.058

Author Ladewig, Katharina
Niebert, Marcus
Xu, Zhi P.
Gray, Peter P.
Lu, Gao Q. M.
Title Efficient siRNA delivery to mammalian cells using layered double hydroxide nanoparticles
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
Publication date 2010-03
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2009.10.058
Volume 31
Issue 7
Start page 1821
End page 1829
Total pages 9
Editor D.F. Williams
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Subject C1
Formatted abstract
Although siRNAs have surpassed expectations in experiments to alter gene expression in vitro, the lack of an efficient in vivo delivery system still remains a challenge in siRNA therapeutics development and has been recognized as a major hurdle for clinical applications. In this paper we describe an inorganic nanoparticle-based delivery system that is readily adaptable for in vivo systems. Layered double hydroxide (LDH) nanoparticles, a family of inorganic crystals, tightly bind, protect, and release siRNA molecules and deliver them efficiently to mammalian cells in vitro. The uptake of siRNA-loaded LDH nanoparticles occurs via endocytosis, whereby the nanoparticles dissolve due to the low pH in the endosome, thereby aiding endosomal escape into the cytoplasm. The influence of LDH nanoparticles on cell viability and proliferation is negligible at concentrations ≤0.050 mg mL-1, and a pronounced downregulation of protein expression upon LDH mediated siRNA transfection of HEK293T cells is observed.
© 2009 Elsevier Ltd. All rights reserved.
Keyword siRNA delivery
Layered double hydroxide nanoparticles
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 17 November 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 77 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 88 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 10 May 2010, 13:44:40 EST by Julie Osborne on behalf of Aust Institute for Bioengineering & Nanotechnology