Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha

Nguyen, Vu Tuan, Flanagan, Bernadine, Mikkelsen, Deirdre, Ramirez, Santiago, Rivas, Lucia, Gidley, Michael J. and Dykes, Gary A. (2010) Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha. Carbohydrate Polymers, 80 2: 337-343. doi:10.1016/j.carbpol.2009.11.019


Author Nguyen, Vu Tuan
Flanagan, Bernadine
Mikkelsen, Deirdre
Ramirez, Santiago
Rivas, Lucia
Gidley, Michael J.
Dykes, Gary A.
Title Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha
Formatted title
Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha
Journal name Carbohydrate Polymers   Check publisher's open access policy
ISSN 0144-8617
1879-1344
Publication date 2010-04-12
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.carbpol.2009.11.019
Volume 80
Issue 2
Start page 337
End page 343
Total pages 7
Editor J. F. Kennedy
J. R. Mitchell
Y.-J. Wang
Place of publication London, U.K
Publisher Applied Science/Elsevier
Collection year 2011
Language eng
Formatted abstract
This study aimed to investigate the characteristics of a spontaneous mutation that results in lower cellulose production by a Gluconacetobacter xylinus strain isolated from Kombucha. The growth of this mutant was initially observed in Hestrin Schramm (HS) static and agitated cultures but not in modified (higher nutrient) HS cultures, indicating different nutrient conditions may play a role in the selection of the spontaneous mutant. The mutant produced a significantly (P < .05) lower amount of cellulose than the wild-type in HS static culture. Nuclear magnetic resonance and scanning electron microscopy indicated that the cellulose produced by the mutant had the same crystalline structure as that produced by the wild-type but had a lower density of fibrils. Two-dimensional gel electrophoresis of total proteins demonstrated the presence of the enzyme deoxythymidine diphosphate (dTDP)-4-dehydrorhamnose 3,5-epimerase in the mutant but not in the wild-type. This enzyme could be involved in the synthesis of acetan, which may reduce cellulose synthesis and be the basis for the characteristics of the mutant.
© 2010 Published by Elsevier Ltd.
Keyword Gluconacetobacter xylinus
Bacterial cellulose
Mutation
Acetobacter-xylinum
Bacterial cellulose
Negative mutants
C-13 NMR
Biosynthesis
Culture
Conversion
Acetan
(13)C NMR
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 20 November 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
Centre for Nutrition and Food Sciences Publications
 
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Created: Sun, 09 May 2010, 00:05:00 EST