Chronic maternal hypertension affects placental gene expression and differentiation in rabbits

McArdle, Adelle M., Maduwegedera, Devaki, Moritz, Karen, Flower, Rebecca L., Denton, Kate M. and Roberts, Claire T. (2010) Chronic maternal hypertension affects placental gene expression and differentiation in rabbits. Journal of Hypertension, 28 5: 959-968. doi:10.1097/HJH.0b013e3283369f1e


Author McArdle, Adelle M.
Maduwegedera, Devaki
Moritz, Karen
Flower, Rebecca L.
Denton, Kate M.
Roberts, Claire T.
Title Chronic maternal hypertension affects placental gene expression and differentiation in rabbits
Journal name Journal of Hypertension   Check publisher's open access policy
ISSN 0263-6352
1473-5598
0952-1178
Publication date 2010-05
Sub-type Article (original research)
DOI 10.1097/HJH.0b013e3283369f1e
Volume 28
Issue 5
Start page 959
End page 968
Total pages 10
Place of publication London, United Kingdom
Publisher Lippincott Williams & Wilkins
Collection year 2011
Language eng
Formatted abstract
Background:
Previously, we have shown that adult offspring from hypertensive rabbits develop hypertension.

Method:
We aimed to determine the effects of mild (+15 mmHg) and moderate (+25 mmHg) increases in maternal blood pressure and plasma renin activity on placental differentiation and expression of components of the renin-angiotensin system and 11 beta-hydroxysteroid dehydrogenase type 2 mRNA in rabbits. Placentas were collected from normotensive ( sham), mild (2-kidney-1-cellophane wrapped; 2K-1W) and moderate (2-kidney-2-cellophane wrapped; 2K-2W) hypertensive groups at gestational age of 14, 21 and 28 days. Placental gene expression was quantified by reverse transcriptase-PCR, and morphometry was assessed by videoimage analyses of placental sections.

Results:
Fetal weight was similar between groups across gestation. In the 2K-1W group at gestational age day 14, fetal-to-placental weight ratio was increased (~34%) as were volumes of fetal capillaries ([up arrow]56%) and maternal blood space at gestational age day 21 ([up arrow]55%) compared with sham (all P < 0.05). In the 2K-2W group, fetal-to-placental weight ratio was increased at gestational age day 21 (~25%; P < 0.01) with an accompanying reduction in placental weight, and at gestational age day 28, volume density of fetal capillaries was increased (~22%; P < 0.05). Placental renin mRNA was lower in both the 2K-1W (~88%) and 2K-2W (~98%) groups at gestational age day 28 (all P < 0.01). Placental 11[beta]-hydroxysteroid dehydrogenase type 2 mRNA was lower in the 2K-1W (~36%) and 2K-2W (~31%) groups at gestational age day 14 and greater (~36%) in the 2K-2W group at gestational age day 21 (all P < 0.01). Associations between placental AT1R and AT2R mRNA and placental differentiation were disturbed by hypertension.

Conclusion:
Mild and moderate maternal hypertension differentially alters placental structure and gene expression that may affect placental functional capacity and contribute to programming of hypertension in offspring.
Copyright: © 2010 Lippincott Williams & Wilkins, Inc.
Keyword Chronic hypertension
Placenta
Pregnancy
Programming
Rabbit
Renin-angiotensin system
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biomedical Sciences Publications
 
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Created: Sun, 02 May 2010, 00:04:56 EST