Protein arginine methyltransferase 6 regulates multiple aspects of gene expression

Harrison, Matthew J., Tang, Yue Hang and Dowhan, Dennis H. (2010) Protein arginine methyltransferase 6 regulates multiple aspects of gene expression. Nucleic Acids Research, 38 7: 2201-2216. doi:10.1093/nar/gkp1203

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Author Harrison, Matthew J.
Tang, Yue Hang
Dowhan, Dennis H.
Title Protein arginine methyltransferase 6 regulates multiple aspects of gene expression
Journal name Nucleic Acids Research   Check publisher's open access policy
ISSN 0305-1048
Publication date 2010-04
Sub-type Article (original research)
DOI 10.1093/nar/gkp1203
Open Access Status DOI
Volume 38
Issue 7
Start page 2201
End page 2216
Total pages 16
Place of publication Oxford, United Kindom
Publisher Oxford University Press
Collection year 2011
Language eng
Formatted abstract
It is well established that transcription and alternative splicing events are functionally coupled during gene expression. Here, we report that protein arginine N-methyltransferase 6 (PRMT6) may play a key role in this coupling process by functioning as a transcriptional coactivator that can also regulate alternative splicing. PRMT6 coactivates the progesterone, glucocorticoid and oestrogen receptors in luciferase reporter assays in a hormone-dependent manner. In addition, small interfering RNA (siRNA) oligonucleotide duplex knockdown of PRMT6 disrupts oestrogen-stimulated transcription of endogenous GREB1 and progesterone receptor in MCF-7 breast cancer cells, demonstrating the importance of PRMT6 in hormone-dependent transcription. In contrast, the regulation of alternative splicing by PRMT6 is hormone independent. siRNA knockdown of PRMT6 increases the exon inclusion: skipping ratio of alternatively spliced exons in endogenous vascular endothelial growth factor and spleen tyrosine kinase RNA transcripts in both the presence and absence of oestrogen. These results demonstrate that PRMT6 has a dual role in regulating gene expression and that these two functions can occur independently of each other.
Keyword Nuclear receptor coactivator
Endothelial growth-factor
Breast-cancer cells
Histone H3
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
UQ Diamantina Institute - Open Access Collection
UQ Diamantina Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 29 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 30 times in Scopus Article | Citations
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Created: Sun, 02 May 2010, 00:02:27 EST