Nature of alpha and beta particles in glycogen using molecular size distributions

Sullivan, Mitchell A., Vilaplana, Francisco, Cave, Richard A., Stapleton, David, Gray-Weale, Angus A. and Gilbert, Robert G. (2010) Nature of alpha and beta particles in glycogen using molecular size distributions. Biomacromolecules, 11 4: 1094-1100. doi:10.1021/bm100074p

Author Sullivan, Mitchell A.
Vilaplana, Francisco
Cave, Richard A.
Stapleton, David
Gray-Weale, Angus A.
Gilbert, Robert G.
Title Nature of alpha and beta particles in glycogen using molecular size distributions
Formatted title
Nature of α and β particles in glycogen using molecular size distributions
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
Publication date 2010-04-12
Sub-type Article (original research)
DOI 10.1021/bm100074p
Volume 11
Issue 4
Start page 1094
End page 1100
Total pages 7
Place of publication Washington, United States
Publisher American Chemical Society
Collection year 2011
Language eng
Formatted abstract
Glycogen is a randomly hyperbranched glucose polymer. Complex branched polymers have two structural levels:
individual branches and the way these branches are linked. Liver glycogen has a third level: supramolecular
clusters of β particles which form larger clusters of α particles. Size distributions of native glycogen were
characterized using size exclusion chromatography (SEC) to find the number and weight distributions and the
size dependences of the number- and weight-average masses. These were fitted to two distinct randomly joined
reference structures, constructed by random attachment of individual branches and as random aggregates of β
particles. The z-average size of the α particles in dimethylsulfoxide does not change significantly with high
concentrations of LiBr, a solvent system that would disrupt hydrogen bonding. These data reveal that the β particles
are covalently bonded to form α particles through a hitherto unsuspected enzyme process, operative in the liver
on particles above a certain size range.  © 2010 American Chemical Society
Keyword Gel-permeation chromatography
Exclusion chromatography
Universal calibration
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
Centre for Nutrition and Food Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 25 Apr 2010, 00:06:32 EST