The utility of neonatal dried blood spots for the assessment of neonatal vitamin D status

Eyles, Darryl W., Morley, Ruth, Anderson, Cameron, Ko, Pauline, Burne, Thomas, Permezel, Michael, Mortensen, Preben B., Nørgaard-Pedersen, Bent, Hougaard, David M. and McGrath, John J. (2010) The utility of neonatal dried blood spots for the assessment of neonatal vitamin D status. Paediatric and Perinatal Epidemiology, 24 3: 303-308. doi:10.1111/j.1365-3016.2010.01105.x

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Author Eyles, Darryl W.
Morley, Ruth
Anderson, Cameron
Ko, Pauline
Burne, Thomas
Permezel, Michael
Mortensen, Preben B.
Nørgaard-Pedersen, Bent
Hougaard, David M.
McGrath, John J.
Title The utility of neonatal dried blood spots for the assessment of neonatal vitamin D status
Journal name Paediatric and Perinatal Epidemiology   Check publisher's open access policy
ISSN 0269-5022
Publication date 2010-05
Sub-type Article (original research)
DOI 10.1111/j.1365-3016.2010.01105.x
Volume 24
Issue 3
Start page 303
End page 308
Total pages 6
Editor J. Golding
T. J. Peters
Place of publication Oxford, U.K
Publisher Blackwell Science
Collection year 2011
Language eng
Formatted abstract
Evidence suggests that low concentrations of 25-hydroxyvitamin D3 (25OHD3) during gestation may be associated with a range of adverse health outcomes in later life. Retrospective estimation of perinatal vitamin D status using questionnaires is extremely unreliable and stored serum samples are rarely available. We aimed to validate the use of dried blood spots (DBS) to estimate perinatal vitamin D status and to determine whether inter-group differences in cord serum 25OHD3 are reflected in DBS. We examined 25OHD3 in 4-year-old frozen cord sera and matched DBS from neonates born at a hospital in Melbourne, Australia (n = 100). We examined the correlation between these values and also investigated whether the expected seasonal (winter/spring vs. summer/autumn) difference in serum 25OHD3 was reflected in DBS values. 25OHD3 was assayed in triplicate using tandem mass spectroscopy in both a 3 µL sample of cord serum and in matched 3 mm punches from archived DBS.

25OHD3 concentrations in neonatal cord serum and DBS were highly correlated (r = 0.85, P < 0.0001). As expected, serum 25OHD3 concentrations were higher in neonates born in summer/autumn (December to March) vs. winter/spring (April to November) (median 46.6 vs. 23.7 nmol/L, P < 0.0001). A comparable difference was seen in DBS values (17.8 vs. 10.5 nmol/L, P = 0.0001). Archived DBS samples provided a valid measure of perinatal vitamin D status and identified inter-seasonal differences in perinatal 25OHD3 concentrations. They could be used for case–control studies investigating the association between perinatal vitamin D status and later health outcomes.
© 2010 Blackwell Publishing Ltd.
Keyword D deficiency
D insufficiency
High prevalence
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Queensland Brain Institute Publications
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 34 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 37 times in Scopus Article | Citations
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Created: Sun, 25 Apr 2010, 00:01:17 EST