A Phospho-Directed Macroporous Alumina-Silica Nanoreactor with Multi-Functions

Qian, K, Wan, JJ, Liu, F, Girault, HH, Liu, BH and Yu, CZ (2009) A Phospho-Directed Macroporous Alumina-Silica Nanoreactor with Multi-Functions. ACS NANO, 3 11: 3656-3662. doi:10.1021/nn900739z


Author Qian, K
Wan, JJ
Liu, F
Girault, HH
Liu, BH
Yu, CZ
Title A Phospho-Directed Macroporous Alumina-Silica Nanoreactor with Multi-Functions
Journal name ACS NANO   Check publisher's open access policy
ISSN 1936-0851
Publication date 2009-11-01
Year available 2009
Sub-type Article (original research)
DOI 10.1021/nn900739z
Volume 3
Issue 11
Start page 3656
End page 3662
Total pages 7
Place of publication United States
Publisher American Chemical Society
Collection year 2010
Language eng
Subject C1
070108 Sustainable Agricultural Development
970109 Expanding Knowledge in Engineering
Abstract A phospho-directed nanoreactor with multiple functions is reported. Alumina-functionalized macroporous ordered silica foams (Al-MOSF) have been developed with large pore size, high pore volume (1.6 cm(3)/g), and a surface area of 186 m(2)/g rich in coordination unsaturated Al species, which can be used as phospho-directed nanoreactors for integrated in situ digestion and in situ phosphoisolation. By directly adding Al-MOSF to the conventional in-solution digestion system, both enzymes and proteins are quickly enriched in the macropores of the reactor to achieve a fast proteolysis without increasing the enzyme/protein concentration or using a preimmobilization process, thus the digestion time and the cost can be greatly reduced. Meanwhile, due to the chemo-affinity between alumina and phosphor groups, the AI-MOSF reactor can in situ isolate specific products of the enzymatic reaction (i.e., phosphopeptides) and release the nonspecific peptides to the solution. This strategy is simple, efficient, and successfully applied in the detection of phosphoproteins in real samples.
Keyword phosphoprotein
macroporous
alumina-silica
nanoreactor
digestion
SPECTROMETRY-BASED PROTEOMICS
HIGHLY EFFICIENT PROTEOLYSIS
ENZYME IMMOBILIZATION
SELECTIVE ENRICHMENT
TITANIUM-DIOXIDE
ROOM-TEMPERATURE
DRUG-DELIVERY
REACTOR
PEPTIDES
PROTEIN
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Chemical Engineering Publications
 
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Created: Fri, 23 Apr 2010, 22:40:23 EST by Amanda Lee on behalf of School of Chemical Engineering