Metabolic engineering of monoterpene biosynthesis: two-step production of (+)-trans-isopiperitenol by tobacco

Lücker, Joost, Schwab, Wilfried, Franssen, Maurice C. R., van der Plas, Linus H. W., Bouwmeester, Harro J. and Verhoeven, Harrie A. (2004) Metabolic engineering of monoterpene biosynthesis: two-step production of (+)-trans-isopiperitenol by tobacco. The Plant Journal, 39 1: 135-145. doi:10.1111/j.1365-313X.2004.02113.x


Author Lücker, Joost
Schwab, Wilfried
Franssen, Maurice C. R.
van der Plas, Linus H. W.
Bouwmeester, Harro J.
Verhoeven, Harrie A.
Title Metabolic engineering of monoterpene biosynthesis: two-step production of (+)-trans-isopiperitenol by tobacco
Formatted title
Metabolic engineering of monoterpene biosynthesis: two-step production of (+)-trans-isopiperitenol by tobacco
Journal name The Plant Journal   Check publisher's open access policy
ISSN 0960-7412
1365-313X
Publication date 2004-07
Sub-type Article (original research)
DOI 10.1111/j.1365-313X.2004.02113.x
Volume 39
Issue 1
Start page 135
End page 145
Total pages 11
Place of publication London, U.K.
Publisher Wiley-Blackwell Publishing
Language eng
Subject 0607 Plant Biology
Abstract Monoterpenoid biosynthesis in tobacco was modified by introducing two subsequent enzymatic activities targeted to different cell compartments. A limonene-3-hydroxylase (lim3h) cDNA was isolated from Mentha spicata L. 'Crispa'. This cDNA was used to re-transform a transgenic Nicotiana tabacum'Petit Havana' SR1 (tobacco) line expressing three Citrus limon L. Burm. f. (lemon) monoterpene synthases producing (+)-limonene, γ-terpinene and (−)-β-pinene as their main products. The targeting sequences of these synthases indicate that they are probably localized in the plastids, whereas the sequence information of the P450 hydroxylase indicates targeting to the endoplasmatic reticulum. Despite the different location of the enzymes, the introduced P450 hydroxylase proved to be functional in the transgenic plants as it hydroxylated (+)-limonene, resulting in the emission of (+)-trans-isopiperitenol. Some further modifications of the (+)-trans-isopiperitenol were also detected, resulting in the additional emission of 1,3,8-p-menthatriene, 1,5,8-p-menthatriene, p-cymene and isopiperitenone. Copyright 2004 Blackwell Publishing Ltd
Keyword Cytochrome P450
Mentha spicata
Metabolic engineering
Monoterpene biosynthesis
Tobacco
(+)-Trans-isopiperitenol
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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