Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (−)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries

Lücker, Joost, Bowen, Pat and Bohlmann, Jörg (2004) Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (−)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries. Phytochemistry, 65 19: 2649-2659. doi:10.1016/j.phytochem.2004.08.017


Author Lücker, Joost
Bowen, Pat
Bohlmann, Jörg
Title Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (−)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries
Formatted title
Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (−)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries
Journal name Phytochemistry   Check publisher's open access policy
ISSN 0031-9422
Publication date 2004-10
Sub-type Article (original research)
DOI 10.1016/j.phytochem.2004.08.017
Volume 65
Issue 19
Start page 2649
End page 2659
Total pages 11
Place of publication Oxford, U.K.
Publisher Pergamon Press
Language eng
Subject 0607 Plant Biology
Formatted abstract
Valencene is a volatile sesquiterpene emitted from flowers of grapevine, Vitis vinifera L. A full-length cDNA from the cultivar Gewürztraminer was functionally expressed in Escherichia coli and found to encode valencene synthase (VvVal). The two major products formed by recombinant VvVal enzyme activity with farnesyl diphosphate (FPP) as substrate are (+)-valencene and (−)-7-epi-α-selinene. Grapevine valencene synthase is closely related to a second sesquiterpene synthase from this species, (−)-germacrene D synthase (VvGerD). VvVal and VvGerD cDNA probes revealed strong signals in Northern hybridizations with RNA isolated from grapevine flower buds. Transcript levels were lower in open pre-anthesis flowers, flowers after anthesis, or at early onset of fruit development. Similar results were obtained using a third probe, (−)-α-terpineol synthase, a monoterpenol synthase. Sesquiterpene synthase and monoterpene synthase transcripts were not detected in the mesocarp and exocarp during early stages of fruit development, but transcripts hybridizing with VvVal appeared during late ripening of the berries. Sesquiterpene synthase transcripts were also detected in young seeds.
Copyright © 2004 Elsevier Ltd All rights reserved.

Keyword Floral scent
Grape vine
Nootkatone
Nutraceuticals
Terpene cyclase
Vitis vinifera
Vitaceae
Wine flavour and aroma
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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