BMP-11 and Myostatin Support Undifferentiated Growth of Human Embryonic Stem Cells in Feeder-Free Cultures

Hannan, N.R.F., Jamshidi, P., Pera, M.F. and Wolvetang, E.J. (2009) BMP-11 and Myostatin Support Undifferentiated Growth of Human Embryonic Stem Cells in Feeder-Free Cultures. Cloning and Stem Cells, 11 3: 427-435. doi:10.1089/clo.2009.0024


Author Hannan, N.R.F.
Jamshidi, P.
Pera, M.F.
Wolvetang, E.J.
Title BMP-11 and Myostatin Support Undifferentiated Growth of Human Embryonic Stem Cells in Feeder-Free Cultures
Journal name Cloning and Stem Cells   Check publisher's open access policy
ISSN 1536-2302
Publication date 2009-09
Year available 2009
Sub-type Article (original research)
DOI 10.1089/clo.2009.0024
Volume 11
Issue 3
Start page 427
End page 435
Total pages 8
Editor Ian Wilmut
Place of publication New Rochelle, NY, USA
Publisher Mary Ann Liebert Inc Publishers
Collection year 2010
Language eng
Subject C1
Abstract BMP-11/GDF-11 and Myostatin/GDF-8 are both members of the TGF-beta superfamily that can activate SMAD2/3 phosphorylation via the type I receptors ALK4, ALK5, or ALK7. We tested the ability of BMP-11 and Myostatin to promote self-renewal of human embryonic stem cells (hESC) under feeder-free and serum-free culture conditions in short term (1 week) and medium term cultures (10 weeks). We show that hESC cultured in serum-free medium supplemented with either 20 ng/mL Myostatin or 20 ng/mL BMP-11 maintain the colony and cellular morphology of undifferentiated hESC, maintain POU5f1, NANOG, TRA-1-60, and SSEA4 expression, and display increased SMAD2/3 phosphorylation, similar to hESC cultured in mouse embryonic fibroblast feeder-CM or 20 ng/mL Activin-A. The type I TGF-beta receptor inhibitor SB431542 totally inhibited the maintenance activity of both Myostatin or BMP-11 supplemented medium. Our data show that members of the TGF-beta superfamily, other than Activin-A and GDF3, are able to maintain hES cells in an undifferentiated state under feeder free conditions.
Keyword MAINTAIN PLURIPOTENCY
SELF-RENEWAL
PATHWAYS
RECEPTORS
SIGNALS
LINES
GDF11
FATE
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Journal name changed from Cloning and Stem Cells to Cellular Reprogramming in 2010.

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 22 Apr 2010, 10:33:15 EST by Sharon Paterson on behalf of Aust Institute for Bioengineering & Nanotechnology