Sterile protection ,against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: Comparison of heterologous prime boost strategies

Jiang, George, Shi, Meng, Conteh, Solomon, Richie, Nancy, Banania, Glenna, Geneshan, Harini, Valencia, Anais, Singh, Priti, Aguiar, Joao, Limbach, Keith, Kamrud, Kurt I., Rayner, Jonathan, Smith, Jonathan, Bruder, Joseph T., King, C. Richter, Tsuboi, Takafumi, Takeo, Satoru, Endo, Yaeta, Doolan, Denise L., Richie, Thomas L. and Weiss, Walter R. (2009) Sterile protection ,against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: Comparison of heterologous prime boost strategies. PloS one, 4 8: e6559 -1-e6559 -12. doi:10.1371/journal.pone.0006559


Author Jiang, George
Shi, Meng
Conteh, Solomon
Richie, Nancy
Banania, Glenna
Geneshan, Harini
Valencia, Anais
Singh, Priti
Aguiar, Joao
Limbach, Keith
Kamrud, Kurt I.
Rayner, Jonathan
Smith, Jonathan
Bruder, Joseph T.
King, C. Richter
Tsuboi, Takafumi
Takeo, Satoru
Endo, Yaeta
Doolan, Denise L.
Richie, Thomas L.
Weiss, Walter R.
Title Sterile protection ,against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: Comparison of heterologous prime boost strategies
Journal name PloS one   Check publisher's open access policy
ISSN 1932-6203
Publication date 2009-08
Sub-type Article (original research)
DOI 10.1371/journal.pone.0006559
Open Access Status DOI
Volume 4
Issue 8
Start page e6559 -1
End page e6559 -12
Total pages 12
Editor Christopher Surridge
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2010
Language eng
Subject C1
92 Health
11 Medical and Health Sciences
Abstract Using newer vaccine platforms which have been effective against malaria in rodent models, we tested five immunization regimens against Plasmodium knowlesi in rhesus monkeys. All vaccines included the same four P. knowlesi antigens: the pre-erythrocytic antigens CSP, SSP2, and erythrocytic antigens AMA1, MSP1. We used four vaccine platforms for prime or boost vaccinations: plasmids (DNA), alphavirus replicons (VRP), attenuated adenovirus serotype 5 (Ad), or attenuated poxvirus (Pox). These four platforms combined to produce five different prime/boost vaccine regimens: Pox alone, VRP/Pox, VRP/Ad, Ad/Pox, and DNA/Pox. Five rhesus monkeys were immunized with each regimen, and five Control monkeys received a mock vaccination. The time to complete vaccinations was 420 days. All monkeys were challenged twice with 100 P. knowlesi sporozoites given IV. The first challenge was given 12 days after the last vaccination, and the monkeys receiving the DNA/Pox vaccine were the best protected, with 3/5 monkeys sterilely protected and 1/5 monkeys that self-cured its parasitemia. There was no protection in monkeys that received Pox malaria vaccine alone without previous priming. The second sporozoite challenge was given 4 months after the first. All 4 monkeys that were protected in the first challenge developed malaria in the second challenge. DNA, VRP and Ad5 vaccines all primed monkeys for strong immune responses after the Pox boost. We discuss the high level but short duration of protection in this experiment and the possible benefits of the long interval between prime and boost.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Article number e6559

Document type: Journal Article
Sub-type: Article (original research)
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Created: Wed, 21 Apr 2010, 13:55:12 EST by Jon Stekhoven on behalf of School of Medicine