Bipolar cells of the mouse retina: A gene gun, morphological study

Pignatelli, Vincenzo and Strettoi, Enrica (2004) Bipolar cells of the mouse retina: A gene gun, morphological study. Journal of Comparative Neurology, 476 3: 254-266. doi:10.1002/cne.20207


Author Pignatelli, Vincenzo
Strettoi, Enrica
Title Bipolar cells of the mouse retina: A gene gun, morphological study
Journal name Journal of Comparative Neurology   Check publisher's open access policy
ISSN 0021-9967
1096-9861
Publication date 2004-08-23
Sub-type Article (original research)
DOI 10.1002/cne.20207
Volume 476
Issue 3
Start page 254
End page 266
Total pages 13
Place of publication Hoboken, NJ, U.S.A.
Publisher John Wiley & Sons
Language eng
Subject 0603 Evolutionary Biology
1109 Neurosciences
Formatted abstract
One of the key elements concerning our understanding of the organization of the mouse retina is the complete classification of the various types of bipolar cells. With the present study, we tried to contribute to this important issue. Unfortunately, most of the antibodies that stain specifically bipolar cells in the retina of other mammals hardly work for the retina of the mouse. We succeeded in overcoming this limitation by using a relatively novel technique based on the gene gun transfer of fluorescent dyes to cells. Hence, we were able to stain a considerable number of bipolar cells that could be characterized according to morphological and comparative criteria. We also performed a complete morphometric analysis of a subset of bipolar cells stained by anti-neurokinin-3 receptor antibodies. We found nine types of cone bipolar cells and one type of rod bipolar cell; these data are consistent with the findings of previous studies on the retinas of other mammals, such as rabbits, rats, and monkeys and with a recent study based on the mouse retina (Ghosh et al. [[2004]] J Comp Neurol 469:70-82). Our results also confirm the existence of a common structural similarity among mammalian retinas. It remains to be elucidated what is exactly the functional role of the various types of cone bipolar cells and what is the specific contribution they provide to the perception of a given visual stimulus. Most probably, each bipolar cell type constitutes a specialized channel for the computation of a selected component of the visual stimulus. More complex signal coding, involving the coordinated activity of various types of bipolar cells, could also be postulated, as it has been shown for ganglion cells (Meister [1996] Proc Natl Acad Sci U S A 93:609-614).
© 2004 Wiley-Liss, Inc.
Keyword Bipolar cells
Gene gun
Morphology
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Queensland Brain Institute Publications
 
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Created: Tue, 20 Apr 2010, 09:25:42 EST by Jon Swabey on behalf of Queensland Brain Institute