Activation of several key components of the epidermal differentiation pathway in cattle following infestation with the cattle tick, Rhipicephalus (Boophilus) microplus

Kongsuwan, K, Josh, P, Colgrave, ML, Bagnall, NH, Gough, J, Burns, B and Pearson, R (2010) Activation of several key components of the epidermal differentiation pathway in cattle following infestation with the cattle tick, Rhipicephalus (Boophilus) microplus. International Journal for Parasitology, 40 4: 499-507. doi:10.1016/j.ijpara.2009.10.013


Author Kongsuwan, K
Josh, P
Colgrave, ML
Bagnall, NH
Gough, J
Burns, B
Pearson, R
Title Activation of several key components of the epidermal differentiation pathway in cattle following infestation with the cattle tick, Rhipicephalus (Boophilus) microplus
Journal name International Journal for Parasitology   Check publisher's open access policy
ISSN 0020-7519
1879-0135
Publication date 2010-03
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.ijpara.2009.10.013
Volume 40
Issue 4
Start page 499
End page 507
Total pages 9
Place of publication Kidlington, Oxford, United Kingdom
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
The cattle tick, Rhipicephalus (Boophilus) microplus, and the diseases it transmits pose a persistent threat to tropical beef production. Genetic selection of host resistance has become the method of choice for non-chemical control of cattle tick. Previous studies have suggested that larval stages are most susceptible to host resistance mechanisms. To gain insights into the molecular basis of host resistance that occurs during R. microplus attachment, we assessed the abundance of proteins (by isobaric tag for relative and absolute quantitation (iTRAQ) and Western blot analyses) and mRNAs (by quantitative reverse transcription PCR (qRT-PCR)) in skin adjacent to tick bite sites from high tick-resistant (HR) and low tick-resistant (LR) Belmont Red cattle following challenge with cattle tick. We showed substantially higher expression of the basal epidermal keratins KRT5 and KRT14, the lipid processing protein, lipocalin 9 (LCN9), the epidermal barrier catalysing enzyme transglutaminase 1 (TGM1), and the transcriptional regulator B lymphocyte-induced maturation protein 1 (Blimp1) in HR skin. Our data reveals the essential role of the epidermal permeability barrier in conferring greater resistance of cattle to tick infestation, and suggest that the physical structure of the epidermal layers of the skin may represent the first line of defence against ectoparasite invasion.
Crown copyright © 2009 Published by Elsevier Ltd.

Keyword Rhipicephalus microplus
Skin
Host resistance
Bovine
Epidermis
Attachment
Gene expression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online November 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Queensland Alliance for Agriculture and Food Innovation
School of Veterinary Science Publications
 
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Created: Sun, 18 Apr 2010, 00:04:59 EST