Speciation of Gram-positive bacteria in fresh and ambient-stored sub-tropical marine fish

Al Bulushi, Ismail M., Poole, Susan E., Barlow, Robert, Deeth, Hilton C. and Dykes, Gary A. (2010) Speciation of Gram-positive bacteria in fresh and ambient-stored sub-tropical marine fish. International Journal of Food Microbiology, 138 1-2: 32-38. doi:10.1016/j.ijfoodmicro.2009.11.021


Author Al Bulushi, Ismail M.
Poole, Susan E.
Barlow, Robert
Deeth, Hilton C.
Dykes, Gary A.
Title Speciation of Gram-positive bacteria in fresh and ambient-stored sub-tropical marine fish
Journal name International Journal of Food Microbiology   Check publisher's open access policy
ISSN 0168-1605
1879-3460
Publication date 2010-03-31
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.ijfoodmicro.2009.11.021
Volume 138
Issue 1-2
Start page 32
End page 38
Total pages 7
Editor L. Cocolin
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Formatted abstract
This study identified Gram-positive bacteria in three sub-tropical marine fish species; Pseudocaranx dentex (silver trevally), Pagrus auratus (snapper) and Mugil cephalus (sea mullet). It further elucidated the role played by fish habitat, fish body part and ambient storage on the composition of the Gram-positive bacteria. A total of 266 isolates of Gram-positive bacteria were identified by conventional biochemical methods, VITEK, PCR using genus- and species-specific primers and/or 16S rRNA gene sequencing. The isolates were found to fall into 13 genera and 30 species. In fresh fish, Staphylococcus epidermidis and Micrococcus luteus were the most frequent isolates. After ambient storage, S. epidermidis, S. xylosus and Bacillus megaterium were no longer present whereas S. warneri, B. sphaericus, Brevibacillus borstelensis, Enterococcus faecium and Streptococcus uberis increased in frequency. Micrococcus luteus and S. warneri were the most prevalent isolates from P. dentex, while E. faecium and Strep. uberis were the most frequent isolates from P. auratus and M. cephalus. With respect to different parts of the fish body, E. faecium, Strep. uberis and B. sphaericus were the most frequent isolates from the muscles, E. faecium, Strep. uberis from the gills and M. luteus from the gut. This study showed a diversity of Gram-positive bacteria in sub-tropical marine fish; however, their abundance was affected by fish habitat, fish body part and ambient storage.
© 2009 Elsevier B.V. All rights reserved.
Keyword Gills
Gram-positive bacteria
Gut
Muscles
Sub-tropical marine fish
Ambient storage
Lactic-acid bacteria
DNA-based assays
Staphylococcus-aureus
Rapid identification
Tropical fish
Spoilage
Water
Microorganisms
Diversity
Listeria
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 4 December 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Agriculture and Food Sciences
 
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Created: Sun, 18 Apr 2010, 00:04:42 EST