Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae

Wu, Hsing-Ju, Seib, Kate L., Srikhanta, Yogitha N., Edwards, Jennifer, Kidd, Stephen P., Maguire,Tina L., Hamilton, Amanda, Pan, Kuan-Tin, Hsiao, He-Hsuan, Yao, Chen-Wen, Grimmond, Sean M., Apicella, Michael A., McEwan, Alastair G., Wang, Andrew H-J. and Jennings, Michael P. (2010) Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae. Journal of Proteomics, 73 5: 899-916. doi:10.1016/j.jprot.2009.12.001


Author Wu, Hsing-Ju
Seib, Kate L.
Srikhanta, Yogitha N.
Edwards, Jennifer
Kidd, Stephen P.
Maguire,Tina L.
Hamilton, Amanda
Pan, Kuan-Tin
Hsiao, He-Hsuan
Yao, Chen-Wen
Grimmond, Sean M.
Apicella, Michael A.
McEwan, Alastair G.
Wang, Andrew H-J.
Jennings, Michael P.
Title Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae
Formatted title
Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae
Journal name Journal of Proteomics   Check publisher's open access policy
ISSN 1874-3919
1876-7737
Publication date 2010-03-01
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.jprot.2009.12.001
Volume 73
Issue 5
Start page 899
End page 916
Total pages 18
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
Neisseria gonorrhoeae has evolved a complex and novel network of oxidative stress responses, including defence mechanisms that are dependent on manganese (Mn). We performed systematic analyses at the transcriptomic and proteomic (1D SDS-PAGE and Isotope-Coded Affinity Tag [ICAT]) levels to investigate the global expression changes that take place in a high Mn environment, which results in a Mn-dependent oxidative stress resistance phenotype. These studies revealed that there were proteins regulated at the post-transcriptional level under conditions of increased Mn concentration, including proteins involved in virulence (e.g., pilin, a key adhesin), oxidative stress defence (e.g., superoxide dismutase), cellular metabolism, protein synthesis, RNA processing and cell division. Mn regulation of inorganic pyrophosphatase (Ppa) indicated the potential involvement of phosphate metabolism in the Mn-dependent oxidative stress defence. A detailed analysis of the role of Ppa and polyphosphate kinase (Ppk) in the gonococcal oxidative stress response revealed that ppk and ppa mutant strains showed increased resistance to oxidative stress. Investigation of these mutants grown with high Mn suggests that phosphate and pyrophosphate are involved in Mn-dependent oxidative stress resistance.
Keyword Manganese
Microarray
Neisseria gonorrhoeae
Oxidative stress
Pili
Proteomics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 11 December 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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Created: Sun, 18 Apr 2010, 10:04:32 EST