Development of a multi-locus sequence typing scheme for avian isolates of Pasteurella multocida

Subaaharan, S., Blackall, L. L. and Blackall, P. J. (2010) Development of a multi-locus sequence typing scheme for avian isolates of Pasteurella multocida. Veterinary Microbiology, 141 3-4: 354-361. doi:10.1016/j.vetmic.2010.01.017


Author Subaaharan, S.
Blackall, L. L.
Blackall, P. J.
Title Development of a multi-locus sequence typing scheme for avian isolates of Pasteurella multocida
Formatted title
Development of a multi-locus sequence typing scheme for avian isolates of Pasteurella multocida
Journal name Veterinary Microbiology   Check publisher's open access policy
ISSN 0378-1135
1873-2542
Publication date 2010-03-24
Sub-type Article (original research)
DOI 10.1016/j.vetmic.2010.01.017
Volume 141
Issue 3-4
Start page 354
End page 361
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Formatted abstract
A total of 63 isolates of Pasteurella multocida from Australian poultry, all associated with
fowl cholera outbreaks, and three international reference strains, representing the three
subspecies within P. multocida were used to develop a multi-locus sequence typing
scheme. Primers were designed for conserved regions of seven house-keeping enzymes –
adk, est, gdh, mdh, pgi, pmi and zwf – and internal fragments of 570–784 bp were sequenced
for all isolates and strains. The number of alleles at the different loci ranged from 11 to 20
and a total of 29 allelic profiles or sequence types were recognised amongst the 66 strains.
There was a strong concordance between the MLST data and the existing multi-locus
enzyme electrophoresis and ribotyping data. When used to study a sub-set of isolates with
a known detailed epidemiological history, the MLST data matched the results given by
restriction endonuclease analysis, pulsed-field gel electrophoresis, ribotyping and REPPCR.
The MLST scheme provides a high level of resolution and is an excellent tool for
studying the population structure and epidemiology of P. multocida.
Crown Copyright 2010 Published by Elsevier B.V. All rights reserved.
Keyword Pasteurella multocida
Multi-locus sequence typing
Avian
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Queensland Alliance for Agriculture and Food Innovation
School of Chemistry and Molecular Biosciences
 
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Created: Sun, 18 Apr 2010, 00:00:32 EST