Nanoscale presentation of cell adhesive molecules via block copolymer self-assembly

George, P.A., Doran, M.R., Croll, T.I., Munro, T.P. and Cooper-White, J.J. (2009) Nanoscale presentation of cell adhesive molecules via block copolymer self-assembly. BIOMATERIALS, 30 27: 4732-4737. doi:10.1016/j.biomaterials.2009.05.039


Author George, P.A.
Doran, M.R.
Croll, T.I.
Munro, T.P.
Cooper-White, J.J.
Title Nanoscale presentation of cell adhesive molecules via block copolymer self-assembly
Journal name BIOMATERIALS   Check publisher's open access policy
ISSN 0142-9612
Publication date 2009-09
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2009.05.039
Volume 30
Issue 27
Start page 4732
End page 4737
Total pages 6
Editor D.F. Williams
Place of publication Netherlands
Publisher Elsevier B V
Collection year 2010
Language eng
Subject C1
Abstract Precise control over the nanoscale presentation of adhesion molecules and other biological factors represents a new frontier for biomaterials science. Recently, the control of integrin spacing and cellular shape has been shown to affect fundamental biological processes, such as differentiation and apoptosis. Here, we present the self-assembly of maleimide functionalised polystyrene-block-poly (ethylene oxide) copolymers as a simple, yet highly precise method for controlling the position of cellular adhesion molecules. By manipulating the phase separation of the functional PS-PEO block copolymer used in this study, via a simple blending technique, we alter the nanoscale (on PEO domains of 8-14 nm in size) presentation of the adhesion peptide, GRGDS, decreasing lateral spacing from 62 nm to 44 nm and increasing the number density from similar to 450 to similar to 900 islands per mu m(2). The results indicate that the spreading of NIH-3T3 fibroblasts increases as the spacing between domains of RGD binding peptides decreases. Further, the same functional PS-PEO surfaces have been utilised to immobilise, via a zinc chelating peptide sequence, poly-histidine tagged proteins and extracellular matrix (ECM) fragments. This method is seen as an ideal platform for investigations into the role of spatial arrangements of cell adhesion molecules and ECM molecules on cell function and, in particular, control of cell phenotype. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
Keyword Nanotechnology
Integrin
Surface modification
Cell spreading
RGD
SURFACES
ADSORPTION
PROTEINS
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 15 Apr 2010, 16:01:29 EST by Sharon Paterson on behalf of Aust Institute for Bioengineering & Nanotechnology