Characterization of endogenous calcium responses in neuronal cell lines

Vetter, Irina and Lewis, Richard J. (2010) Characterization of endogenous calcium responses in neuronal cell lines. Biochemical Pharmacology, 79 6: 908-920. doi:10.1016/j.bcp.2009.10.020

Author Vetter, Irina
Lewis, Richard J.
Title Characterization of endogenous calcium responses in neuronal cell lines
Journal name Biochemical Pharmacology   Check publisher's open access policy
ISSN 0006-2952
Publication date 2010-03-15
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.bcp.2009.10.020
Volume 79
Issue 6
Start page 908
End page 920
Total pages 13
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2011
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
030505 Physical Organic Chemistry
Formatted abstract
An increasing number of putative therapeutic targets have been identified in recent years for the treatment of neuronal pathophysiologies including pain, epilepsy, stroke and schizophrenia. Many of these targets signal through calcium (Ca2+), either by directly facilitating Ca2+ influx through an ion channel, or through activation of G proteins that couple to intracellular Ca2+ stores or voltage-gated Ca2+ channels. Immortalized neuronal cell lines are widely used models to study neuropharmacology. However, systematic pharmacological characterization of the receptors and ion channels expressed in these cell lines is lacking. In this study, we systematically assessed endogenous Ca2+ signaling in response to addition of agonists at potential therapeutic targets in a range of cell lines of neuronal origin (ND7/23, SH-SY5Y, 50B11, F11 and Neuro2A cells) as well as HEK293 cells, a cell line commonly used for over-expression of receptors and ion channels. This study revealed a remarkable diversity of endogenous Ca2+ responses in these cell lines, with one or more cell lines responding to addition of trypsin, bradykinin, ATP, nicotine, acetylcholine, histamine and neurotensin. Subtype specificity of these responses was inferred from agonist potency and the effect of receptor subtype specific antagonist. Surprisingly, HEK293 and SH-SY5Y cells responded to the largest number of agonists with potential roles in neuronal signaling. These findings have implications for the heterologous expression of neuronal receptors and ion channels in these cell lines, and highlight the potential of neuron-derived cell lines for the study of a range of endogenously expressed receptors and ion channels that signal through Ca2+.
Copyright © 2011 Elsevier B.V.
Keyword Endogenous
Neuronal cell line
Ion channel
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article published online October 31, 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 30 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
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Created: Thu, 15 Apr 2010, 11:30:21 EST by Susan Allen on behalf of Institute for Molecular Bioscience