Enhanced human bone marrow stromal cell affinity for modified poly(L-lactide) surfaces by the upregulation of adhesion molecular genes

Mao, X.L., Peng, H., Ling, J.Q., Friis, T., Whittaker, A.K., Crawford, R. and Xiao, Y. (2009) Enhanced human bone marrow stromal cell affinity for modified poly(L-lactide) surfaces by the upregulation of adhesion molecular genes. BIOMATERIALS, 30 36: 6903-6911.


Author Mao, X.L.
Peng, H.
Ling, J.Q.
Friis, T.
Whittaker, A.K.
Crawford, R.
Xiao, Y.
Title Enhanced human bone marrow stromal cell affinity for modified poly(L-lactide) surfaces by the upregulation of adhesion molecular genes
Journal name BIOMATERIALS  (ERA 2012 Listed)    (ERA 2010 Rank A*)   Check publisher's open access policy
Publication date 2009-12
Sub-type Article
Year available 2009
DOI 10.1016/j.biomaterials.2009.09.013
Volume number 30
Issue number 36
ISSN 0142-9612
Start page 6903
End page 6911
Total pages 9
Editor D.F. Williams
Place of publication Netherlands
Publisher Elsevier B.V.
Collection year 2010
Language eng
Subject C1
Abstract To enhance and regulate cell affinity for Poly (L-lactic acid) (PLLA) based materials, two hydrophilic ligands, poly (ethylene glycol) (PEG) and poly (L-lysine) (PLL), were used to develop triblock copolymers: methoxy-terminated poly (ethylene glycol)-block-poly (L-lactide)-block-poly (L-lysine) (MPEG-b-PLLA-b-PLL) in order to regulate protein absorption and cell adhesion. Bone marrow stromal cells (BMSCs) were cultured on different composition of MPEG-b-PLLA-b-PLL copolymer films to determine the effect of modified polymer surfaces on BMSC attachment. To understand the molecular mechanism governing the initial cell adhesion on difference polymer surfaces, the mRNA expression of 84 human extracellular matrix (ECM) and adhesion molecules was analysed using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). It was found that down regulation of adhesion molecules was responsible for the impaired BMSC attachment on PLIA surface. MPEG-b-PLLA-b-PLL copolymer films improved significantly the cell adhesion and cytoskeleton expression by upregulation of relevant molecule genes significantly. Six adhesion genes (CDH1, ITGL, NCAM1, SGCE, COL16A1, and LAMA3) were most significantly influenced by the modified PLLA surfaces. In summary, polymer surfaces altered adhesion molecule gene expression of BMSCs, which consequently regulated cell initial attachment on modified PLLA surfaces. (C) 2009 Elsevier Ltd. All rights reserved.
Keyword Bone marrow
Stem cell
Cell adhesion
Polylactic acid
Adhesion mechanism
Gene expression
PROTEIN ADSORPTION
POLY(ETHYLENE GLYCOL)
RGD PEPTIDE
ATTACHMENT
COPOLYMERS
TISSUE
GLYCOL)-B-POLY(L-LACTIDE)-B-POLY(L-LYSINE)
BIOMATERIALS
NETWORKS
COATINGS
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Tue, 13 Apr 2010, 16:10:38 EST by Sandrine Ducrot on behalf of Aust Institute for Bioengineering & Nanotechnology