Identification of genes differentially expressed by prematurely fused human sutures using a novel in vivo-in vitro approach

Coussens, A. K., Hughes, I. P., Wilkinson, C. R., Morris, P., Anderson, P. J., Powell, B. C. and van Daal, A. (2008) Identification of genes differentially expressed by prematurely fused human sutures using a novel in vivo-in vitro approach. Differentiation, 76 5: 531-545. doi:10.1111/j.1432-0436.2007.00244.x


Author Coussens, A. K.
Hughes, I. P.
Wilkinson, C. R.
Morris, P.
Anderson, P. J.
Powell, B. C.
van Daal, A.
Title Identification of genes differentially expressed by prematurely fused human sutures using a novel in vivo-in vitro approach
Journal name Differentiation   Check publisher's open access policy
ISSN 0301-4681
1432-0436
Publication date 2008-06
Sub-type Article (original research)
DOI 10.1111/j.1432-0436.2007.00244.x
Volume 76
Issue 5
Start page 531
End page 545
Total pages 14
Publisher Elsevier
Language eng
Subject 06 Biological Sciences
0601 Biochemistry and Cell Biology
Abstract Craniosynostosis is the premature fusion of calvarial sutures. It results from abnormal differentiation or proliferation of cells within the osteogenic fronts of growing calvarial bones. To date, research has focused on animal models and in vitro organ and tissue culture to determine the molecular mechanisms controlling calvarial suture morphogenesis. Here, we test a new, in vivo–in vitro approach based on the hypothesis that calvarial suture cells passaged in minimal medium exhibit a stable gene expression profile similar to undifferentiated osteoblastic cells that can provide a benchmark for comparison with in vivo expression of differentiated tissue. We show that tissue-specific expression is lost after the first passage and, using cDNA microarrays, compare expression between fused suture tissue from craniosynostosis patients and in vitro de-differentiated explant cells. A large number of differentially expressed genes were identified, including novel genes WIF1, LEF1, SATB2, RARRES1, DEFA1, DMP1, PTPRZ1, and PTPRC, as well as those commonly associated with human suture morphogenesis, e.g., FGF2, MSX2, and BMP2. Two differentially expressed genes, WIF1 and FGF2, were further examined in an in vivo–in vivo comparison between unfused and prematurely fused tissue. The same pattern of differential expression was observed in each case, further validating the ability of our in vivo–in vitro approach to identify genes involved in in vivo human calvarial tissue differentiation.
Keyword Craniosynostosis
Microarray
Differentiation
Osteoblast
De-differentiate
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 12 Apr 2010, 13:27:57 EST by Therese Egan on behalf of Faculty Of Health Sciences