Fluorescent layered double hydroxide nanoparticles for biological studies

Musumeci, Anthony W., Mortimer, Gysell M., Butler, Margaret K., Xu, Zhi Ping, Minchin, Rodney M. and Martin, Darren J. (2010) Fluorescent layered double hydroxide nanoparticles for biological studies. Applied Clay Science, 48 1-2: 271-279. doi:10.1016/j.clay.2009.11.008

Author Musumeci, Anthony W.
Mortimer, Gysell M.
Butler, Margaret K.
Xu, Zhi Ping
Minchin, Rodney M.
Martin, Darren J.
Title Fluorescent layered double hydroxide nanoparticles for biological studies
Journal name Applied Clay Science   Check publisher's open access policy
ISSN 0169-1317
Publication date 2010-03
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.clay.2009.11.008
Open Access Status
Volume 48
Issue 1-2
Start page 271
End page 279
Total pages 9
Editor G J Churchman
G Legaly
Place of publication Netherlands
Publisher Elsevier BV
Collection year 2010
Language eng
Subject C1
970103 Expanding Knowledge in the Chemical Sciences
1007 Nanotechnology
Abstract Layered double hydroxide (LDH) nanoparticles have the potential to benefit a myriad of medical, consumer and industrial products if intricate control of the nanoscale architecture and understanding of the biological interactions and potential toxicity of the LDH nanomaterials are achieved. Here, we report the synthesis and fractionation of LDH nanomaterials of well defined spatial characteristics through the careful control of synthesis conditions and the use of ultracentrifugation. The appropriateness of a range of fluorescent dyes, for labelling LDH nanoparticles to enable biological tracking, has also been investigated. These dyes include fluorescein, fluorescein isothiocyanate (FITC), 8-aminonaphthelene-1,3,6-trisulfonic acid (ANTS) and 8-aminopyrene-1,3,6-trisulfonic acid (APTS). Thermal degradation of the popular FITC dye during nanoparticle synthesis was revealed and suitable alternative fluorescent molecules established. Furthermore, we report for the first time the application of size exclusion chromatography on LDH nanoparticles as a fast, reliable and efficient method to achieve purification without causing nanoparticle agglomeration.
Keyword Layered double hydroxide nanoparticles
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 25 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 11 Apr 2010, 00:10:10 EST