FMRFamide gene and peptide expression during central nervous system development of the cephalopod mollusk, Idiosepius notoides

Wollesen, Tim, Cummins, Scott F., Degnan, Bernard M. and Wanninger, Andreas (2010) FMRFamide gene and peptide expression during central nervous system development of the cephalopod mollusk, Idiosepius notoides. Evolution and Development, 12 2: 113-130. doi:10.1111/j.1525-142X.2010.00398.x


Author Wollesen, Tim
Cummins, Scott F.
Degnan, Bernard M.
Wanninger, Andreas
Title FMRFamide gene and peptide expression during central nervous system development of the cephalopod mollusk, Idiosepius notoides
Formatted title
FMRFamide gene and peptide expression during central nervous system development of the cephalopod mollusk, Idiosepius notoides
Journal name Evolution and Development   Check publisher's open access policy
ISSN 1520-541X
1525-142X
Publication date 2010-03
Sub-type Article (original research)
DOI 10.1111/j.1525-142X.2010.00398.x
Volume 12
Issue 2
Start page 113
End page 130
Total pages 8
Editor Rudolf A. Raff
Place of publication Malden, MA, U.S.A.
Publisher Blackwell Science
Collection year 2011
Language eng
Subject C1
Formatted abstract
Mollusks are a showcase of brain evolution represented by several classes with a varying degree of nervous system centralization. Cellular and molecular processes involved in the evolution of the highly complex cephalopod brain from a simple, monoplacophoran-like ancestor are still obscure and homologies on the cellular level are poorly established. FMRFamide (Phe-Ile-Arg-Phe-NH2)-related peptides (FaRPs) constitute an evolutionarily conserved and diverse group of neuropeptides in the central nervous system (CNS) of many metazoans. Herein, we provide a detailed description of the developing FMRFamide-like immunoreactive (Fa-lir) CNS of the pygmy squid Idiosepius notoides using gene expression analyses and immunocytochemistry. The open reading frame of the I. notoides FMRFamide gene InFMRF predicts one copy each of FIRFamide, FLRFamide (Phe-Leu-Arg-Phe-NH2), ALSGDAFLRFamide (Ala-Leu-Ser-Gly-Asp-Ala-Phe-Leu-Arg-Phe-NH2), and 11 copies of FMRFamide. Applying matrix-assisted laser desorption/ionization time-of-flight (ToF) mass spectrometry-based peptide profiling, we characterized all predicted FaRPs except ALSGDAFLRFamide. Two cell clusters express InFMRF and show FMRFamide-like-immunoreactivity within the palliovisceral ganglia, that is, the future posterior subesophageal mass, during the lobe differentiation phase. They project neurites via ventral axonal tracts, which form the scaffold of the future subesophageal mass. In the supraesophageal mass, InFMRF is first expressed during mid-embryogenesis in the superior and inferior buccal lobes. A neurite of the peduncle commissure represents the first Fa-lir element. Later, the sub- and supraesophageal mass interconnect via Fa-lir neurites and more brain lobes express InFMRF and FMRFamidelike peptides. InFMRF expression was observed in fewer brain lobes than Fa-lir elements. The early expression of InFMRF and FMRFamide-lir peptides in the visceral system and not the remaining CNS of the cephalopod I. notoides resembles the condition found in the majority of investigated gastropods.
© 2010 Wiley Periodicals, Inc.
Keyword Cuttlefish sepia-officinalis
Snail lymnaea-stagnalis
Neuropeptide gene
Octopus-vulgaris
Optic gland
Drosophila-melanogaster
Insitu hybidization
Aplysi-californica
Todarodes-pacificus
Identified neurons
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes March/April 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biological Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 28 times in Scopus Article | Citations
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Created: Sun, 11 Apr 2010, 00:06:25 EST