Conservation of the egg-laying hormone neuropeptide and attractin pheromone in the spotted sea hare, Aplysia dactylomela

Cummins, Scott F., Nuurai, Parinyaporn, Nagle, Gregg T. and Degnan, Bernard M. (2010) Conservation of the egg-laying hormone neuropeptide and attractin pheromone in the spotted sea hare, Aplysia dactylomela. Peptides, 31 3: 394-401. doi:10.1016/j.peptides.2009.10.010


Author Cummins, Scott F.
Nuurai, Parinyaporn
Nagle, Gregg T.
Degnan, Bernard M.
Title Conservation of the egg-laying hormone neuropeptide and attractin pheromone in the spotted sea hare, Aplysia dactylomela
Formatted title
Conservation of the egg laying hormone neuropeptide and attractin pheromone in the spotted sea hare, Aplysia dactylomela.
Journal name Peptides   Check publisher's open access policy
ISSN 0196-9781
Publication date 2010-03
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.peptides.2009.10.010
Volume 31
Issue 3
Start page 394
End page 401
Total pages 8
Place of publication New York, NY, United States
Publisher Elsevier Science
Collection year 2011
Language eng
Subject 830103 Aquaculture Molluscs (excl. Oysters)
060604 Comparative Physiology
C1
Formatted abstract
In the marine opisthobranchmollusc, Aplysia, secreted peptides and proteins play an essential role in egg laying and mate attraction. Aplysia californica egg laying is initiated by secretion of the egg-laying hormone (ELH) peptide while mate attraction is made possible by protein pheromones, such as attractin, released into the surrounding seawater with the egg cordon. In this study, we investigated the existence of similar egg-laying hormone and attractin products in the spotted sea hare, Aplysia dactylomela, a species that is widely distributed in almost all tropical and temperate oceans, including Australia’s Great Barrier Reef. Immunological analysis revealed that an ELH-like transmitter is present within bag cell somata and processes of the abdominal ganglion. A molecular genetic approach found that the ELH precursor mRNA is synthesized in the abdominal ganglia and encodes a 36-residue peptide (dELH) that is cleaved from the prohormone prior to secretion. It is most closely related to A. californica and A. brasiliana ELH (91.7% identical). We also found that A. dactylomela synthesize an attractin pheromone in the albumen gland that is released during egg laying. The gene encodes a 58-residue mature protein that is 74.9% similar to A. californica attractin. We demonstrate that an increase in seawater temperature can disrupt attractins higher order interactions, such as those with the pheromone temptin, and accelerates attractin degradation. Together, these findings further expands our understanding of pheromone intermolecular interactions and presents an opportunity for further study of how increases in sea water temperature may affect this important marine communication system.
Copyright © 2009 Elsevier Inc. All rights reserved.
Keyword Aplysia
Egg-laying hormone
Attractin
Pheromone
Temperature
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Special issue Invertebrate Neuropeptides X. Available online 23 October 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Degnan Laboratory
2010 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Sun, 11 Apr 2010, 00:01:40 EST