Keratinocytes efficiently process endogenous antigens for cytotoxic T-cell mediated lysis

Zhou, F, Frazer, IH and Leggatt, GR (2009) Keratinocytes efficiently process endogenous antigens for cytotoxic T-cell mediated lysis. EXPERIMENTAL DERMATOLOGY, 18 12: 1053-1059. doi:10.1111/j.1600-0625.2009.00907.x

Author Zhou, F
Frazer, IH
Leggatt, GR
Title Keratinocytes efficiently process endogenous antigens for cytotoxic T-cell mediated lysis
Journal name EXPERIMENTAL DERMATOLOGY   Check publisher's open access policy
ISSN 0906-6705
Publication date 2009-12
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1600-0625.2009.00907.x
Volume 18
Issue 12
Start page 1053
End page 1059
Total pages 7
Editor Paus, R.
Place of publication United Kingdom
Publisher Wiley - Blackwell Publishing Ltd
Collection year 2010
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
970111 Expanding Knowledge in the Medical and Health Sciences
920102 Cancer and Related Disorders
920108 Immune System and Allergy
920109 Infectious Diseases
1107 Immunology
1112 Oncology and Carcinogenesis
Abstract The generation of a robust CD8(+) cytotoxic T lymphocyte (CTL) response is a key feature of many immunotherapeutic strategies against epithelial tumors and virally infected epithelial tissue. However, surprisingly few studies have addressed whether primary epithelial cells, expressing defined endogenous antigens, are good targets for CTL-mediated lysis. Here, we show that primary keratinocytes (KCs), expressing endogenous ovalbumin (OVA) as a transgene, present measurable H-2K(b)/SIINFEKL complexes at the cell surface and are killed by OVA-specific CTL. Target cell lysis was comparable with a more traditional CTL target cell, EL4, and was enhanced by KC pretreatment with interferon-gamma. These results suggest that primary KCs will be susceptible to CTL-mediated apoptosis when endogenous KC antigens are targeted during immunotherapy.
Keyword cytotoxic T cell
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
UQ Diamantina Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 09 Apr 2010, 15:36:02 EST by Kylie Hengst on behalf of UQ Diamantina Institute