Human papillomavirus DNA detected in peripheral blood samples from healthy Australian male blood donors

Chen, Alice, Keleher, Annie, Kedda, Mary-Anne, Spurdle, Amanda, McMillan, Nigel and Antonsson, Annika (2009) Human papillomavirus DNA detected in peripheral blood samples from healthy Australian male blood donors. Journal of Medical Virology, 81 10: 1792-1796. doi:10.1002/jmv.21592

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Author Chen, Alice
Keleher, Annie
Kedda, Mary-Anne
Spurdle, Amanda
McMillan, Nigel
Antonsson, Annika
Title Human papillomavirus DNA detected in peripheral blood samples from healthy Australian male blood donors
Journal name Journal of Medical Virology   Check publisher's open access policy
ISSN 0146-6615
Publication date 2009-10
Year available 2009
Sub-type Article (original research)
DOI 10.1002/jmv.21592
Open Access Status
Volume 81
Issue 10
Start page 1792
End page 1796
Total pages 5
Editor Zuckerman, A. J.
Place of publication New Jersey, United States
Publisher Wiley-Liss
Collection year 2010
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
970111 Expanding Knowledge in the Medical and Health Sciences
920102 Cancer and Related Disorders
920109 Infectious Diseases
110309 Infectious Diseases
1112 Oncology and Carcinogenesis
Abstract Recent studies have shown that human papillomavirus (HPV) DNA can be found in circulating blood, including peripheral blood mononuclear cells (PBMCs), sera, plasma, and arterial cord blood. In light of these findings, DNA extracted from PBMCs from healthy blood donors were examined in order to determine how common HPV DNA is in blood of healthy individuals. Blood samples were collected from 180 healthy male blood donors (18-76 years old) through the Australian Red Cross Blood Services. Genomic DNA was extracted and specimens were tested for HPV DNA by PCR using a broad range primer pair. Positive samples were HPV-type determined by cloning and sequencing. HPV DNA was found in 8.3% (15/180) of the blood donors. A wide variety of different HPV types were isolated from the PBMCs; belonging to the cutaneous beta and gamma papillomavirus genera and mucosal alpha papillomaviruses. High-risk HPV types that are linked to cancer development were detected in 1.7% (3/180) of the PBMCs. Blood was also collected from a healthy HPV-positive 44-year-old male on four different occasions in order to determine which blood cell fractions harbor HPV. PBMCs treated with trypsin were negative for HPV, while non-trypsinized PBMCs were HPV-positive. This suggests that the HPV in blood is attached to the outside of blood cells via a protein-containing moiety. HPV was also isolated in the B cells, dendritic cells, NK cells, and neutrophils. To conclude, HPV present in PBMCs could represent a reservoir of virus and a potential new route of transmission.
Keyword Squamous-cell carcinoma
Colorectal-cancer
Infection
Receptor
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
UQ Diamantina Institute Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 08 Apr 2010, 16:13:20 EST by Sarah Macaione on behalf of UQ Diamantina Institute