Central nervous system expression of the exon 9 skipping form of the glutamate transporter GLAST

Macnab, Lauren T. and Pow, David V. (2007) Central nervous system expression of the exon 9 skipping form of the glutamate transporter GLAST. NeuroReport, 18 8: 741-745. doi:10.1097/WNR.0b013e3280c143b0


Author Macnab, Lauren T.
Pow, David V.
Title Central nervous system expression of the exon 9 skipping form of the glutamate transporter GLAST
Journal name NeuroReport   Check publisher's open access policy
ISSN 0959-4965
1473-558X
Publication date 2007-05-28
Sub-type Article (original research)
DOI 10.1097/WNR.0b013e3280c143b0
Volume 18
Issue 8
Start page 741
End page 745
Total pages 5
Editor Giorgio Gabella
Place of publication London, U.K.
Publisher Lippincott Williams & Wilkins
Language eng
Subject 1109 Neurosciences
1702 Cognitive Sciences
Formatted abstract
We have raised antibodies that selectively recognize an exon 9 skipping form of GLAST. We demonstrate expression of this protein in brains of rats, cats, monkeys and humans. Immunolabelling was present in scattered populations of neurons, particularly in cerebral cortex and colliculi. Neurons were often present in small clusters and exhibited a range of morphologies, from apparently normal to highly degenerate. GLAST1b was also expressed by some glial cells. Cortical neurons expressing the exon 9 skipping form of GLAST also labelled with antibodies against the C- or N-terminal regions of GLAST. We suggest that alternate splicing of GLAST by subpopulations of neurons may indicate some dysfunction in these cells, and may be an indicator of inappropriate local excitation.
© 2007 Lippincott Williams & Wilkins, Inc.
Keyword Western blotting
Callithrix
Cats
Central nervous system
Excitatory amino acid transporter 1
Gene expression
Immunoblotting
Immunohistochemistry
Mice
Neurons
Protein structure, tertiary
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
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