Echinococcus granulosus: Pre-culture of protoscoleces in vitro significantly increases development and viability of secondary hydatid cysts in mice

Zhang, Wen-Bao, Jones, Malcolm K., Li, Jun and McManus, Donald P. (2005) Echinococcus granulosus: Pre-culture of protoscoleces in vitro significantly increases development and viability of secondary hydatid cysts in mice. Experimental Parasitology, 110 1: 88-90. doi:10.1016/j.exppara.2005.02.003


Author Zhang, Wen-Bao
Jones, Malcolm K.
Li, Jun
McManus, Donald P.
Title Echinococcus granulosus: Pre-culture of protoscoleces in vitro significantly increases development and viability of secondary hydatid cysts in mice
Journal name Experimental Parasitology   Check publisher's open access policy
ISSN 0014-4894
Publication date 2005-05
Sub-type Article (original research)
DOI 10.1016/j.exppara.2005.02.003
Volume 110
Issue 1
Start page 88
End page 90
Total pages 3
Place of publication United States
Publisher Elsevier; Academic Press
Language eng
Subject 06 Biological Sciences
0605 Microbiology
Abstract We describe a method for obtaining improved secondary infections of Echinococcus granulosus that involves culturing protoscolex larvae in vitro prior to inoculation into mice. This approach provides a far superior method for obtaining secondary echinococcosis infections in mice compared with the traditional method of direct inoculation of protoscoleces (PSC) where the majority of parasites are killed by the host. We obtained a high rate of recovery both in terms of secondary cyst numbers and their viability. After 50 weeks post-infection (p.i.), brood capsules were formed and the first PSC developed in each of the capsules. After 56 weeks p.i., the fastest developing brood capsule contained four PSC. The approach will prove valuable for investigating parasite development and the host–parasite interaction in secondary echinococcosis.
Keyword Echinococcus granulosus
Secondary echinococcosis
Hydatid cyst development
In vitro culture
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Public Health Publications
 
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Created: Wed, 31 Mar 2010, 11:31:32 EST by Therese Egan on behalf of Faculty Of Health Sciences