The evolution of Runx genes II. The C-terminal Groucho recruitment motif is present in both eumetazoans and homoscleromorphs but absent in a haplosclerid demosponge

Robertson, Anthony J., Larroux, Claire, Degnan, Bernard M. and Coffman, James A. (2009) The evolution of Runx genes II. The C-terminal Groucho recruitment motif is present in both eumetazoans and homoscleromorphs but absent in a haplosclerid demosponge. BMC Research Notes, 2 59: 59.1-59.9. doi:10.1186/1756-0500-2-59


Author Robertson, Anthony J.
Larroux, Claire
Degnan, Bernard M.
Coffman, James A.
Title The evolution of Runx genes II. The C-terminal Groucho recruitment motif is present in both eumetazoans and homoscleromorphs but absent in a haplosclerid demosponge
Journal name BMC Research Notes   Check publisher's open access policy
ISSN 1756-0500
Publication date 2009-04
Sub-type Article (original research)
DOI 10.1186/1756-0500-2-59
Open Access Status DOI
Volume 2
Issue 59
Start page 59.1
End page 59.9
Total pages 9
Editor Dr. Melissa Norton
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2010
Language eng
Formatted abstract
Background: The Runt DNA binding domain (Runx) defines a metazoan family of sequencespecific transcription factors with essential roles in animal ontogeny and stem cell based development. Depending on cis-regulatory context, Runx proteins mediate either transcriptional activation or repression. In many contexts Runx-mediated repression is carried out by Groucho/ TLE, recruited to the transcriptional complex via a C-terminal WRPY sequence motif that is found
encoded in all heretofore known Runx genes.
Findings: Full-length Runx genes were identified in the recently sequenced genomes of phylogenetically diverse metazoans, including placozoans and sponges, the most basally branching members of that clade. No sequences with significant similarity to the Runt domain were found in the genome of the choanoflagellate Monosiga brevicollis, confirming that Runx is a metazoan apomorphy. A contig assembled from genomic sequences of the haplosclerid demosponge Amphimedon queenslandica was used to construct a model of the single Runx gene from that species,
AmqRunx, the veracity of which was confirmed by expressed sequences. The encoded sequence of the Runx protein OscRunx from the homoscleromorph sponge Oscarella carmella was also obtained from assembled ESTs. Remarkably, a syntenic linkage between Runx and Supt3h, previously reported in vertebrates, is conserved in A. queenslandica. Whereas OscRunx encodes a C-terminal
Groucho-recruitment motif, AmqRunx does not, although a Groucho homologue is found in the A. queenslandica genome.
Conclusion: Our results are consistent with the hypothesis that sponges are paraphyletic, and suggest that Runx-WRPY mediated recruitment of Groucho to cis-regulatory sequences originated in the ancestors of eumetazoans following their divergence from demosponges.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article # 59

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Created: Tue, 30 Mar 2010, 12:20:05 EST by Hayley Ware on behalf of School of Biological Sciences