H-Cadherin expression reduces invasion of malignant melanoma

Kuphal, Silke, Martyn, Adam C., Pedley, Julie, Crowther, Lisa M., Bonazzi, Vanessa F., Parsons, Peter G., Bosserhoff, Anja K., Hayward, Nicholas K. and Boyle, Glen M. (2009) H-Cadherin expression reduces invasion of malignant melanoma. Pigment Cell and Melanoma Research, 22 3: 296-306. doi:10.1111/j.1755-148X.2009.00568.x


Author Kuphal, Silke
Martyn, Adam C.
Pedley, Julie
Crowther, Lisa M.
Bonazzi, Vanessa F.
Parsons, Peter G.
Bosserhoff, Anja K.
Hayward, Nicholas K.
Boyle, Glen M.
Title H-Cadherin expression reduces invasion of malignant melanoma
Journal name Pigment Cell and Melanoma Research   Check publisher's open access policy
ISSN 1755-1471
1755-148X
Publication date 2009-06
Sub-type Article (original research)
DOI 10.1111/j.1755-148X.2009.00568.x
Volume 22
Issue 3
Start page 296
End page 306
Total pages 11
Editor Colin R. Goding
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell
Collection year 2010
Language eng
Subject C1
970111 Expanding Knowledge in the Medical and Health Sciences
111201 Cancer Cell Biology
Abstract Melanocytic behavior, survival, and proliferation are regulated through a complex system of cell–cell adhesion molecules. Pathologic changes leading to development of malignant melanoma, upset the delicate homeostatic balance between melanocytes and keratinocytes and can lead to altered expression of cell–cell adhesion and cell–cell communication molecules. Malignant transformation of melanocytes frequently coincides with loss of E-cadherin expression. We now show loss of another member of the superfamily of classical cadherins, H-cadherin (CDH13), which may be involved in the development of malignant melanoma. The provided data show that H-cadherin expression is lost in nearly 80% of the analyzed melanoma cell lines. Knockdown of H-cadherin using siRNA increases invasive capacity in melanocytes. Functional assays show that the re-expression of H-cadherin decreases migration and invasion capacity, as well as anchorage-independent growth in comparison to control melanoma cells. Furthermore, melanoma cells, which re-express H-cadherin via stable transfection show a reduction in rate of tumor growth in a nu/nu mouse tumor model in comparison to the parental control transfected cell lines. Our study presents for the first time the down-regulation of H-cadherin in malignant melanomas and its possible functional relevance in maintenance healthy skin architecture.
Formatted abstract


Keyword H-cadherin
Migration
Invasion
Melanocytes
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Medicine Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 29 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 30 Mar 2010, 09:22:43 EST by Amanda Jones on behalf of Medicine - Royal Brisbane and Women's Hospital