Expression profiling identifies genes involved in emphysema severity

Francis, Santiyagu M. Savarimuthu, Larsen, Jill E., Pavey, Sandra J., Bowman, Rayleen V., Hayward, Nicholas K., Fong, Kwun M. and Yang, Ian A. (2009) Expression profiling identifies genes involved in emphysema severity. Respiratory Research, 10 1: 1-10. doi:10.1186/1465-9921-10-81


Author Francis, Santiyagu M. Savarimuthu
Larsen, Jill E.
Pavey, Sandra J.
Bowman, Rayleen V.
Hayward, Nicholas K.
Fong, Kwun M.
Yang, Ian A.
Title Expression profiling identifies genes involved in emphysema severity
Journal name Respiratory Research   Check publisher's open access policy
ISSN 1465-9921
1465-993X
Publication date 2009-09-02
Year available 2009
Sub-type Article (original research)
DOI 10.1186/1465-9921-10-81
Open Access Status DOI
Volume 10
Issue 1
Start page 1
End page 10
Total pages 10
Editor Jan Lotvall
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2010
Language eng
Subject 9201 Clinical Health (Organs, Diseases and Abnormal Conditions)
110203 Respiratory Diseases
Formatted abstract
Chronic obstructive pulmonary disease (COPD) is a major public health problem. The aim of this study was to identify genes involved in emphysema severity in COPD patients.

Gene expression profiling was performed on total RNA extracted from non-tumor lung tissue from 30 smokers with emphysema. Class comparison analysis based on gas transfer measurement was performed to identify differentially expressed genes. Genes were then selected for technical validation by quantitative reverse transcriptase-PCR (qRT-PCR) if also represented on microarray platforms used in previously published emphysema studies. Genes technically validated advanced to tests of biological replication by qRT-PCR using an independent test set of 62 lung samples.

Class comparison identified 98 differentially expressed genes (p < 0.01). Fifty-one of those genes had been previously evaluated in differentiation between normal and severe emphysema lung. qRT-PCR confirmed the direction of change in expression in 29 of the 51 genes and 11 of those validated, remaining significant at p < 0.05. Biological replication in an independent cohort confirmed the altered expression of eight genes, with seven genes differentially expressed by greater than 1.3 fold, identifying these as candidate determinants of emphysema severity.

Gene expression profiling of lung from emphysema patients identified seven candidate genes associated with emphysema severity including COL6A3, SERPINF1, ZNHIT6, NEDD4, CDKN2A, NRN1 and GSTM3.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online 2009 September

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Tue, 30 Mar 2010, 19:09:14 EST by Amanda Jones on behalf of Medicine - Royal Brisbane and Women's Hospital