HIF-1α, HIF-2α and VHL mRNA expression in different cell lines under hypoxia

Bobarykina, A. Yu., Minchenko, D. O., Opentanova, I. L., Kovtun, O. O., Komisarenko, S. V., Esumi, H. and Minchenko, O. H. (2006) HIF-1α, HIF-2α and VHL mRNA expression in different cell lines under hypoxia. Ukrainskii Biokhimicheskii Zhurnal, 78 2: 62-72.


Author Bobarykina, A. Yu.
Minchenko, D. O.
Opentanova, I. L.
Kovtun, O. O.
Komisarenko, S. V.
Esumi, H.
Minchenko, O. H.
Title HIF-1α, HIF-2α and VHL mRNA expression in different cell lines under hypoxia
Journal name Ukrainskii Biokhimicheskii Zhurnal   Check publisher's open access policy
ISSN 0201-8470
Publication date 2006-03
Sub-type Article (original research)
Volume 78
Issue 2
Start page 62
End page 72
Total pages 11
Editor V. K. Lishko
Place of publication Ukraine
Publisher Natsional'na Akademiya Nauk Ukrainy, Instytut Biokhimii im. A.V. Palladina
Language rus
Subject 0601 Biochemistry and Cell Biology
Formatted abstract
Hypoxia inducible factor-1α (HIF-1α) mRNA expression is significantly decreased under hypoxia in different cell lines exposed directly to hypoxia or treated with dimethyloxalylglycine which mimics hypoxic effects under normoxic conditions. However, the decreased expression of HIF-1α mRNA is accompanied by an increase of HIF-1α protein (pHIF-1α) level as well as by overexpression of known HIF-dependent genes (VEGF, Glut1, PFKFB-3 and PFKFB-4) under hypoxic conditions or with the use of dimethyloxalylglycine. Expression of HIF-1α mRNA also depends on iron because desferrioxamine and cobalt chloride produce similar to hypoxia effects on the levels of this mRNA. It was shown that HIF-1α mRNA expression did not change significantly in some cell lines (SKBR3, MDA-MB468 and BT549) under hypoxia. However, in these cell lines hypoxia decreases expression of HIF-2α mRNA, another member of HIF-α gene family, as a result of cell specific regulation of HIF-α genes under hypoxia. Moreover, hypoxia slightly induces expression of PFKFB-4 mRNA in SKBR3, MDA-MB468 and BT549 as compared to other cell lines where this effect of hypoxia was much stronger and adaptation to hypoxia is controlled by HIF-1α. Hypoxia slightly reduces expression of tumor suppressor VHL which targets HIF-1α for ubiquitination. Thus, our results clearly demonstrated down regulation of HIF-1α or HlF-2α in different cell lines by hypoxia.

Keyword Dimethyloxalylglycine
HIF-1α
Hypoxia
VHL
Q-Index Code C1
Additional Notes Issue March-April 2006

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Wed, 24 Mar 2010, 15:19:50 EST by June Temby on behalf of Institute for Molecular Bioscience