Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptides: A fluorescence study using Pep-1

Henriques, Sónia T., Costa, Júlia and Castanho, Miguel A.R.B. (2005) Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptides: A fluorescence study using Pep-1. FEBS letters, 579 20: 4498-4502. doi:10.1016/j.febslet.2005.06.085


Author Henriques, Sónia T.
Costa, Júlia
Castanho, Miguel A.R.B.
Title Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptides: A fluorescence study using Pep-1
Journal name FEBS letters   Check publisher's open access policy
ISSN 0014-5793
1873-3468
Publication date 2005-08-15
Sub-type Article (original research)
DOI 10.1016/j.febslet.2005.06.085
Volume 579
Issue 20
Start page 4498
End page 4502
Total pages 5
Editor F. Wieland
Place of publication The Netherlands
Publisher Elsevier
Language eng
Subject 0601 Biochemistry and Cell Biology
Formatted abstract
Cell-penetrating peptides (CPPs) are able to translocate across biological membranes and deliver bioactive proteins. Cellular uptake and intracellular distribution of CPPs is commonly evaluated with fluorescent labels, which can alter peptide properties. The effect of carboxyfluorescein label in the Lys-rich domain of the amphipathic CPP pep-1, was evaluated and compared with non-labelled pep-1 in vitro and in vivo. A reduced membrane affinity and an endosomal-dependent translocation mechanism, at variance with non-labelled pep-1, were detected. Therefore, the charged domain is not a mere enabler of peptide adsorption but has a crucial role in the translocation pathway of non-labelled pep-1.
Copyright © 2005 Federation of European Biochemical Societies Published by Elsevier B.V.

Keyword Cell-penetrating peptide
Drug delivery
Translocation mechanism
Transmembrane potential
Q-Index Code C1
Additional Notes Article edited by Peter Brzezinski

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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