Transcriptional changes in Schistosoma mansoni during early schistosomula development and in the presence of erythrocytes

Gobert, Geoffrey N., Tran, Mai H., Moertel, Luke, Mulvenna, Jason, Jones, Malcolm K., McManus, Donald P. and Loukas, Alex (2010) Transcriptional changes in Schistosoma mansoni during early schistosomula development and in the presence of erythrocytes. Plos Neglected Tropical Diseases, 4 2: e600 -1-e600 -13. doi:10.1371/journal.pntd.0000600


Author Gobert, Geoffrey N.
Tran, Mai H.
Moertel, Luke
Mulvenna, Jason
Jones, Malcolm K.
McManus, Donald P.
Loukas, Alex
Title Transcriptional changes in Schistosoma mansoni during early schistosomula development and in the presence of erythrocytes
Formatted title
Transcriptional changes in Schistosoma mansoni during early schistosomula development and in the presence of erythrocytes
Journal name Plos Neglected Tropical Diseases   Check publisher's open access policy
ISSN 1935-2735
Publication date 2010-02
Sub-type Article (original research)
DOI 10.1371/journal.pntd.0000600
Open Access Status DOI
Volume 4
Issue 2
Start page e600 -1
End page e600 -13
Total pages 13
Editor Elodie Ghedin
Place of publication San Francisco, United States
Publisher Public Library of Science
Collection year 2011
Language eng
Formatted abstract
Background: Schistosomes cause more mortality and morbidity than any other human helminth, but control primarily relies on a single drug that kills adult worms. The newly transformed schistosomulum stage is susceptible to the immune response and is a target for vaccine development and rational drug design.

Methodology/Principal Findings: To identify genes which are up-regulated during the maturation of Schistosoma mansoni schistosomula in vitro, we cultured newly transformed parasites for 3 h or 5 days with and without erythrocytes and compared their transcriptional profiles using cDNA microarrays. The most apparent changes were in the up-regulation of genes between 3 h and 5 day schistosomula involved in blood feeding, tegument and cytoskeletal development, cell adhesion, and stress responses. The most highly up-regulated genes included a tegument tetraspanin Sm-tsp-3 (1,600-fold up-regulation), a protein kinase, a novel serine protease and serine protease inhibitor, and intestinal proteases belonging to distinct mechanistic classes. The inclusion of erythrocytes in the culture medium resulted in a general but less pronounced increase in transcriptional activity, with the highest up-regulation of genes involved in iron metabolism, proteolysis, and transport of fatty acids and sugars.

Conclusions: We have identified the genes that are up-regulated during the first 5 days of schistosomula development in vitro. Using a combination of gene silencing techniques and murine protection studies, some of these highly up-regulated transcripts can be targeted for future development of new vaccines and drugs.

Keyword Lung-stage larvae
22.6 kds antigen
Gene expression
Microarray analysis
RNA interference
Blood fluke
Japonicum
Parasite
Protein
Surface
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number e600

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 37 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 42 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 21 Mar 2010, 00:01:09 EST