CD36/fatty acid translocase in rats: Distribution, isolation from hepatocytes, and comparison with the scavenger receptor SR-B1

Zhang, Xingqi, Fitzsimmons, Rebecca L., Cleland, Leslie G., Ey, Peter L, Zannettino, Andrew C. W., Farmer, Elizabeth-Anne, Sincock, Paul and Mayrhofer, Graham (2003) CD36/fatty acid translocase in rats: Distribution, isolation from hepatocytes, and comparison with the scavenger receptor SR-B1. Laboratory Investigation, 83 3: 317-332. doi:10.1097/01.LAB.0000059923.67198.BA


Author Zhang, Xingqi
Fitzsimmons, Rebecca L.
Cleland, Leslie G.
Ey, Peter L
Zannettino, Andrew C. W.
Farmer, Elizabeth-Anne
Sincock, Paul
Mayrhofer, Graham
Title CD36/fatty acid translocase in rats: Distribution, isolation from hepatocytes, and comparison with the scavenger receptor SR-B1
Journal name Laboratory Investigation   Check publisher's open access policy
ISSN 0023-6837
Publication date 2003-03
Sub-type Article (original research)
DOI 10.1097/01.LAB.0000059923.67198.BA
Volume 83
Issue 3
Start page 317
End page 332
Total pages 16
Place of publication Baltimore, MD, U.S.A.
Publisher Nature Publishing
Language eng
Subject 1103 Clinical Sciences
Abstract SUMMARY: The new mAb UA009 recognizes an antigen expressed by microvascular endothelium, by lymphatic endothelium, and by some epithelia in a number of organs, including the small intestine, lactating mammary gland, kidney, lung, sebaceous glands, and circumvallate papillae of the tongue. This antigen is also expressed abundantly in the splenic red pulp and marginal zone and by monocytes, macrophages, and erythrocytes (but not by platelets). Among tissues that store or metabolize fatty acids, the antigen is expressed by adipocytes, cardiomyocytes, and red skeletal muscle. Importantly, it is expressed by steroidogenic cells in the adrenal gland, testis, and ovary, whereas in the liver it is expressed by hepatocytes in a pattern that is dependent on gender and genetic background. mAb UA009 immunoprecipitated a mol wt 85-kDa surface protein from detergent extracts of hepatocytes from Dark Agouti female rats. The N-terminal amino acid sequence of this protein was identical to fatty acid translocase (FAT), the rat cluster of differentiation 36 (CD36) ortholog. The mAb also reacted with COS-7 cells transfected with cDNA encoding FAT. cDNAs encoding a CD36/FAT-like polypeptide were prepared from both liver and heart RNA by RT-PCR. The nucleotide sequences obtained from these cDNAs (Dark Agouti rats) revealed identity and 99% similarity, respectively, with the published sequences of Cd36/Fat in rats of the Wistar and Sprague-Dawley strains. The absence of the UA009 antigen in CD36/FAT-deficient SHR/N rats confirmed the identity of the UA009 antigen and CD36/FAT. We suggest that CD36/FAT might function in the liver as a ex-regulated accessory molecule, either in reverse cholesterol transport and/or in fatty acid uptake. (Lab Invest 2003, 83:317–332).
Formatted abstract
T
Keyword Antigen
CD36/FAT
Clinical studies
Laboratory Animals
Rats
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 41 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Fri, 19 Mar 2010, 14:07:43 EST by Ms May Balasaize on behalf of Institute for Molecular Bioscience