Morphological and immunohistochemical characterization of day 21 IVP and NT bovine embryos

Alexopoulos, N. I., Maddox-Hyttel, P., Tecirlioglu, R. T., Cooney, M. A. and French, A. J. (2005). Morphological and immunohistochemical characterization of day 21 IVP and NT bovine embryos. In: Reproduction, Fertility and Development. Proceedings of the Annual Conference of the International Embryo Transfer Society. IETS 31st Annual Conference of the International Embryo Transfer Society, Copenhagen, Denmark,, (163-163). 8-12 January 2005. doi:10.1071/RDv17n2Ab27


Author Alexopoulos, N. I.
Maddox-Hyttel, P.
Tecirlioglu, R. T.
Cooney, M. A.
French, A. J.
Title of paper Morphological and immunohistochemical characterization of day 21 IVP and NT bovine embryos
Conference name IETS 31st Annual Conference of the International Embryo Transfer Society
Conference location Copenhagen, Denmark,
Conference dates 8-12 January 2005
Proceedings title Reproduction, Fertility and Development. Proceedings of the Annual Conference of the International Embryo Transfer Society   Check publisher's open access policy
Place of Publication East Melbourne, Vic., Australia
Publisher CSIRO
Publication Year 2005
Sub-type Poster
DOI 10.1071/RDv17n2Ab27
ISSN 1031-3613
1448-5990
Volume 17
Issue 1 & 2
Start page 163
End page 163
Total pages 1
Language eng
Formatted Abstract/Summary
A major limitation of somatic cell nuclear transfer (NT) for the production of cloned calves is that only 1–5% of cloned embryos produce viable calves. The high rate of mortality is attributed to both pre- and post-natal losses and is primarily due to incomplete reprogramming of donor cells. Almost 50% of the pregnancy losses occur in the first trimester of pregnancy, indicating a major disruption in normal embryo development at NT. The aim of this study was to analyze germ layer formation by stereomicroscopy and immunohistochemical techniques for both NT embryos and their in vivo counterparts on Day 21 and to compare deviations from normal embryonic development as a measure of developmental capacity.

In conclusion, although localization of staining in IVF and cloned embryos was consistent with that of in vivo embryos, the intensity was weaker, suggesting compromised or delayed development. It is also possible that the differences observed were due to the recipient and not to the treatment group.
Subjects 1114 Paediatrics and Reproductive Medicine
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Presented and published as poster no.27 under "Cloning/Nuclear Transfer".

 
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Created: Wed, 17 Mar 2010, 20:39:33 EST by Jon Swabey on behalf of Queensland Brain Institute