A mutation in an Arabidopsis ribose 5-phosphate isomerase reduces cellulose synthesis and is rescued by exogenous uridine

Howles, Paul A., Birch, Rosemary J., Collings, David A., Gebbie, Leigh K., Hurley, Ursula A., Hocart, Charles H., Arioli, Tony and Williamson, Richard E. (2006) A mutation in an Arabidopsis ribose 5-phosphate isomerase reduces cellulose synthesis and is rescued by exogenous uridine. The Plant Journal, 48 4: 606-618. doi:10.1111/j.1365-313X.2006.02902.x

Author Howles, Paul A.
Birch, Rosemary J.
Collings, David A.
Gebbie, Leigh K.
Hurley, Ursula A.
Hocart, Charles H.
Arioli, Tony
Williamson, Richard E.
Title A mutation in an Arabidopsis ribose 5-phosphate isomerase reduces cellulose synthesis and is rescued by exogenous uridine
Journal name The Plant Journal   Check publisher's open access policy
ISSN 0960-7412
Publication date 2006-11
Sub-type Article (original research)
DOI 10.1111/j.1365-313X.2006.02902.x
Volume 48
Issue 4
Start page 606
End page 618
Total pages 13
Place of publication Oxford, U.K.
Publisher BIOS Publishers : Blackwell Scientific in association with the Society for Experimental Biology
Language eng
Subject 0607 Plant Biology
Formatted abstract
The Arabidopsis radial swelling mutant rsw10 showed ballooning of root trichoblasts, a lower than wild-type level of cellulose and altered levels of some monosaccharides in non-cellulosic polysaccharides. Map-based cloning showed that the mutated gene (At1g71100) encodes a ribose 5-phosphate isomerase (RPI) and that the rsw10 mutation replaces a conserved glutamic acid residue with lysine. Although RPI is intimately involved with many biochemical pathways, media supplementation experiments suggest that the visible phenotype results from a defect in the production of pyrimidine-based sugar–nucleotide compounds, most likely uridine 5'-diphosphate–glucose, the presumed substrate of cellulose synthase. Two of three RPI sequences in the nuclear genome are cytoplasmic, while the third has a putative chloroplast transit sequence. The sequence encoding both cytoplasmic enzymes could complement the mutation when expressed behind the CaMV 35S promoter, while fusion of the RSW10 promoter region to the GUS reporter gene established that the gene is expressed in many aerial tissues as well as the roots. The prominence of the rsw10 phenotype in roots probably reflects RSW10 being the only cytosolic RPI in this tissue and the gene encoding the plastid RPI being relatively weakly expressed. We could not, however, detect a decrease in total RPI activity in root extracts. The rsw10 phenotype is prominent near the root tip where cells undergo division, endoreduplication and cell expansion and so are susceptible to a restriction in de novo pyrimidine production. The two cytosolic RPIs probably arose in an ancient duplication event, their present expression patterns representing subfunctionalization of the expression of the original ancestral gene.

Keyword Ribose 5-phosphate isomerase
Radial swelling mutant
Cell wall
Pyrimidine biosynthesis
Arabidopsis thaliana
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Wed, 17 Mar 2010, 10:36:53 EST by June Temby on behalf of Aust Institute for Bioengineering & Nanotechnology