Activity assays and immunoassays for plasma renin and prorenin: Information provided and precautions necessary for accurate measurement

Campbell, Duncan J., Nussberger, Juerg, Stowasser, Michael, Danser, A. H. Jan, Morganti, Alberto, Frandsen, Erik and Menard, Joel (2009) Activity assays and immunoassays for plasma renin and prorenin: Information provided and precautions necessary for accurate measurement. Clinical Chemistry, 55 5: 867-877. doi:10.1373/clinchem.2008.118000


Author Campbell, Duncan J.
Nussberger, Juerg
Stowasser, Michael
Danser, A. H. Jan
Morganti, Alberto
Frandsen, Erik
Menard, Joel
Title Activity assays and immunoassays for plasma renin and prorenin: Information provided and precautions necessary for accurate measurement
Journal name Clinical Chemistry   Check publisher's open access policy
ISSN 0009-9147
1530-8561
Publication date 2009-03-05
Year available 2009
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1373/clinchem.2008.118000
Volume 55
Issue 5
Start page 867
End page 877
Total pages 11
Place of publication Washington, DC, U.S.A.
Publisher American Association for Clinical Chemistry
Collection year 2010
Language eng
Subject 920106 Endocrine Organs and Diseases (excl. Diabetes)
110306 Endocrinology
Formatted abstract BACKGROUND: Measurement of plasma renin is important for the clinical assessment of hypertensive patients. The most common methods for measuring plasma renin are the plasma renin activity (PRA) assay and the renin immunoassay. The clinical application of renin inhibitor therapy has thrown into focus the differences in information provided by activity assays and immunoassays for renin and prorenin measurement and has drawn attention to the need for precautions to ensure their accurate measurement. 

CONTENT: Renin activity assays and immunoassays provide related but different information. Whereas activity assays measure only active renin, immunoassays measure both active and inhibited renin. Particular care must be taken in the collection and processing of blood samples and in the performance of these assays to avoid errors in renin measurement. Both activity assays and immunoassays are susceptible to renin overestimation due to prorenin activation. In addition, activity assays performed with peptidase inhibitors may overestimate the degree of inhibition of PRA by renin inhibitor therapy. Moreover, immunoassays may overestimate the reactive increase in plasma renin concentration in response to renin inhibitor therapy, owing to the inhibitor promoting conversion of prorenin to an open conformation that is recognized by renin immunoassays. 

CONCLUSIONS: The successful application of renin assays to patient care requires that the clinician and the clinical chemist understand the information provided by these assays and of the precautions necessary to ensure their accuracy. 
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: ERA 2012 Admin Only
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Created: Tue, 16 Mar 2010, 15:01:44 EST by Denise Wilson on behalf of Medicine - Princess Alexandra Hospital