Cell division cycle of the nucleated planctomycete bacterium Gemmata obscuriglobus

Kuo-Chang. Lee and Fuerst, John A. (2008). Cell division cycle of the nucleated planctomycete bacterium Gemmata obscuriglobus. In: 12th International Symposium on Microbial Ecology (ISME 12), Cairns, Australia, (). 17-22 August 2008.

Author Kuo-Chang. Lee
Fuerst, John A.
Title of paper Cell division cycle of the nucleated planctomycete bacterium Gemmata obscuriglobus
Conference name 12th International Symposium on Microbial Ecology (ISME 12)
Conference location Cairns, Australia
Conference dates 17-22 August 2008
Place of Publication Australia
Publisher International Society for Microbial Ecology
Publication Year 2008
Sub-type Poster
Language eng
Abstract/Summary Members of phylum Planctomycetes of the domain Bacteria are distinctive budding peptidoglycan-less and compartmentalized bacteria from aquatic and soil habitats.The planctomycete Gemmata obscuriglobus forms a special model for cell biology since although phylogenetically related to Bacteria, it possesses a eukaryote-like nuclear body in which the nucleoid is enclosed by a double membrane. In this study, phase contrast microscopy was used to study the G. obscuriglobus cell division cycle over time, and fluorescence microscopy and transmission electron microscopy were applied to investigate fate of the nuclear body during this cycle. During the budding process, the bud was formed and increased in size from the polar end of the mother cell until separation.The matured daughter cell acted as a new mother cell and started its own budding cycle while the mother cell can itself initiate budding repeatedly. Fluorescence microscopy demonstrated that translocation of the nucleoid and formation of the bud did not occur at the same time.Electron microscopy revealed that nuclear envelope started to form only after the nucleoid was translocated into the bud. The nuclear body membranes appeared to originate from the intracytoplasmic membrane (ICM). In summary, the division cycle of the nucleated planctomycete G. obscuriglobus appears to be a complex process in which chromosomal DNA is transported to the daughter cell bud after initial formation of the bud, and this can be performed repeatedly by a single mother cell. These results form the basis for further progress at the molecular level to elucidate the developmental biology of this planctomycete model.
Subjects 0303 Macromolecular and Materials Chemistry
03 Chemical Sciences
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Poster number: 86 Presented on Thursday, August 21, 2008, 15:30 – 18:00. Dedicated poster session: Single Cell Microbiology

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Created: Mon, 15 Mar 2010, 12:38:15 EST by Laura McTaggart on behalf of Faculty of Science