Innervation of tracheal parasympathetic ganglia by esophageal cholinergic neurons: evidence from anatomic and functional studies in guinea pigs

Mazzone, Stuart B. and McGovern, Alice E. (2010) Innervation of tracheal parasympathetic ganglia by esophageal cholinergic neurons: evidence from anatomic and functional studies in guinea pigs. American Journal of Physiology - Lung Cellular and Molecular Physiology, 298 3: L404-L416. doi:10.1152/ajplung.00166.2009


Author Mazzone, Stuart B.
McGovern, Alice E.
Title Innervation of tracheal parasympathetic ganglia by esophageal cholinergic neurons: evidence from anatomic and functional studies in guinea pigs
Journal name American Journal of Physiology - Lung Cellular and Molecular Physiology   Check publisher's open access policy
ISSN 1040-0605
1522-1504
Publication date 2010-01
Sub-type Article (original research)
DOI 10.1152/ajplung.00166.2009
Volume 298
Issue 3
Start page L404
End page L416
Total pages 13
Place of publication Bethesda, MD, United States
Publisher American Physiology Society
Collection year 2011
Language eng
Subject C1
920115 Respiratory System and Diseases (incl. Asthma)
060603 Animal Physiology - Systems
Abstract In the present study, we describe a subset of nerve fibers, characterized by their immunoreactivity for the calcium-binding protein calretinin, that are densely and selectively associated with cholinergic postganglionic neurons in the guinea pig tracheal ganglia. Retrograde neuronal tracing with cholera toxin B, combined with immunohistochemical analyses, showed that these nerve fibers do not originate from sensory neurons in the nodose, jugular, or dorsal root ganglia or from motor neurons in the nucleus ambiguus, dorsal motor nucleus of the vagus nerve, spinal cord, stellate ganglia, or superior cervical ganglia. Calretinin-immunoreactive nerve fibers disappeared from tracheal segments after 48 h in organotypic culture, indicating that the fibers were of extrinsic origin. However, calretinin-positive nerve fibers persisted in tracheal ganglia when tracheae were cocultured with the adjacent esophagus intact. Immunohistochemical analysis of the esophagus revealed a population of cholinergic neurons in the esophageal myenteric plexus that coexpressed calretinin. In functional studies, electrical stimulation of the esophagus in vitro evoked measurable contractions of the trachea. These contractions were not altered by prior organotypic culture of the trachea and esophagus to remove the extrinsic innervation to the airways but were significantly (P < 0.05) inhibited by the ganglionic blocker hexamethonium or by physical disruption of the tissue connecting the trachea and esophagus. These data suggest that a subset of esophageal neurons, characterized by the expression of calretinin and acetylcholine, provide a previously unrecognized excitatory input to tracheal cholinergic ganglia in guinea pigs. Copyright © 2010 American Physiological Society.
Keyword Airway innervation
Parasympathetic nervous system
Gastroesophageal reflux disease
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biomedical Sciences Publications
 
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Created: Sun, 14 Mar 2010, 00:08:05 EST