Molecular characterization and distinguishing features of a novel human rhinovirus (HRV) C, HRVC-QCE, detected in children with fever, cough and wheeze during 2003

Arden, K. E., Faux, C. E., O'Neill, N. T., McErlean, P., Nitsche, A., Lambert, S. B., Nissen, M. D., Sloots, T. P. and Mackay, I. M. (2010) Molecular characterization and distinguishing features of a novel human rhinovirus (HRV) C, HRVC-QCE, detected in children with fever, cough and wheeze during 2003. Journal of Clinical Virology, 47 3: 219-223. doi:10.1016/j.jcv.2010.01.001


Author Arden, K. E.
Faux, C. E.
O'Neill, N. T.
McErlean, P.
Nitsche, A.
Lambert, S. B.
Nissen, M. D.
Sloots, T. P.
Mackay, I. M.
Title Molecular characterization and distinguishing features of a novel human rhinovirus (HRV) C, HRVC-QCE, detected in children with fever, cough and wheeze during 2003
Journal name Journal of Clinical Virology   Check publisher's open access policy
ISSN 1386-6532
1873-5967
Publication date 2010-03
Sub-type Article (original research)
DOI 10.1016/j.jcv.2010.01.001
Volume 47
Issue 3
Start page 219
End page 223
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Formatted abstract
Background

Human rhinoviruses (HRVs) are associated with more acute respiratory tract infections than any other viral group yet we know little about viral diversity, epidemiology or clinical outcome resulting from infection by strains, in particular the recently identified HRVs.

Objectives


To determine whether HRVC-QCE was a distinct HRV-C strain, by determining its genome and prevalence, by cataloguing genomic features for strain discrimination and by observing clinical features in positive patients.

Study design

Novel real-time RT-PCRs and retrospective chart reviews were used to investigate a well-defined population of 1247 specimen extracts to observe the prevalence and the clinical features of each HRV-QCE positive case from an in- and out-patient pediatric, hospital-based population during 2003. An objective illness severity score was determined for each HRVC-QCE positive patient.

Results


Differences in overall polyprotein and VP1 binding pocket residues and the predicted presence of a cis-acting replication element in 1B defined HRVC-QCE as a novel HRV-C strain. Twelve additional HRVC-QCE detections (1.0% prevalence) occurred among infants and toddlers (1–24 months) suffering mild to moderate illness, including fever and cough, who were often hospitalized. HRVC-QCE was frequently detected in the absence of another virus and was the only virus detected in three (23% of HRVC-QCE positives) children with asthma exacerbation and in two (15%) toddlers with febrile convulsion.

Conclusions


HRVC-QCE is a newly identified, genetically distinct HRV strain detected in hospitalized children with a range of clinical features. HRV strains should be independently considered to ensure we do not overestimate the HRVs in asymptomatic illness.
Keyword Rhinovirus
Acute respiratory tract infection
HRV C
Wheeze
Respiratory virus epidemiology
Virus discovery
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Chemistry and Molecular Biosciences
Clinical Medical Virology Centre Publications
 
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Created: Sun, 14 Mar 2010, 00:04:01 EST