Metabolic flux analysis in mammalian cell culture

Quek, Lake-Ee, Dietmair, Stefanie, Kromer, Jens O. and Nielsen, Lars K. (2010) Metabolic flux analysis in mammalian cell culture. Metabolic Engineering, 12 2: 161-171. doi:10.1016/j.ymben.2009.09.002

Author Quek, Lake-Ee
Dietmair, Stefanie
Kromer, Jens O.
Nielsen, Lars K.
Title Metabolic flux analysis in mammalian cell culture
Journal name Metabolic Engineering   Check publisher's open access policy
ISSN 1096-7176
Publication date 2010-03
Sub-type Article (original research)
DOI 10.1016/j.ymben.2009.09.002
Volume 12
Issue 2
Start page 161
End page 171
Total pages 11
Editor G. N. Stephanopoulos
Place of publication United States
Publisher Academic Press
Collection year 2010
Language eng
Abstract Mammalian cell culture metabolism is characterized by glucoglutaminolysis, that is, high glucose and glutamine uptake combined with a high rate of lactate and non-essential amino acid secretion. Stress associated with acid neutralization and ammonia accumulation necessitates complex feeding schemes and limits cell densities achieved in fed-batch culture. Conventional and constraint-based metabolic flux analysis has been successfully used to study the metabolic phenotype of mammalian cells in culture, while 13C tracer analysis has been used to study small network models and validate assumptions of metabolism. Large-scale 13C metabolic flux analysis, which is required to improve confidence in the network models and their predictions, remains a major challenge. Advances in both modeling and analytical techniques are bringing this challenge within sight. (C)2009 Elsevier Inc.
Keyword Metabolic flux analysis (MFA)
Mammalian cells
Constrained-based analysis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Sun, 14 Mar 2010, 00:02:12 EST