Suppressive subtractive hybridization analysis of Rhipicephalus (Boophilus) microplus larval and adult transcript expression during attachment and feeding

Lew-Tabor, Ala E., Moolhuijzen, Paula M, Vance, Megan E, Kurscheid, Sebastian, Rodriguez Valle, Manuel, Jarrett, Sandra, Minchin, Catherine M, Jackson, Louise A, Jonsson, Nick N, Bellgard, Matthew I and Guerrero, Felix D (2010) Suppressive subtractive hybridization analysis of Rhipicephalus (Boophilus) microplus larval and adult transcript expression during attachment and feeding. Veterinary Parasitology, 167 2-4: 304-320. doi:10.1016/j.vetpar.2009.09.033

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Author Lew-Tabor, Ala E.
Moolhuijzen, Paula M
Vance, Megan E
Kurscheid, Sebastian
Rodriguez Valle, Manuel
Jarrett, Sandra
Minchin, Catherine M
Jackson, Louise A
Jonsson, Nick N
Bellgard, Matthew I
Guerrero, Felix D
Title Suppressive subtractive hybridization analysis of Rhipicephalus (Boophilus) microplus larval and adult transcript expression during attachment and feeding
Formatted title
Suppressive subtractive hybridization analysis of Rhipicephalus (Boophilus) microplus larval and adult transcript expression during attachment and feeding
Journal name Veterinary Parasitology   Check publisher's open access policy
ISSN 0304-4017
1873-2550
Publication date 2010-02-10
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.vetpar.2009.09.033
Open Access Status
Volume 167
Issue 2-4
Start page 304
End page 320
Total pages 17
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2011
Language eng
Formatted abstract
Ticks, as blood-feeding ectoparasites, affect their hosts both directly and as vectors of viral, bacterial and protozoal diseases. The tick's mode of feeding means it must maintain intimate contact with the host in the face of host defensive responses for a prolonged time. The parasite–host interactions are characterized by the host response and parasite counter-response which result in a highly complex biological system that is barely understood. We conducted transcriptomic analyses utilizing suppressive subtractive hybridization (SSH) to identify transcripts associated with host attachment and feeding of larval, adult female and adult male ticks. Five SSH libraries resulted in 511 clones (assembled into 36 contigs and 90 singletons) from differentially expressed transcripts isolated from unattached frustrated larvae (95), feeding larvae (159), unattached frustrated adult female ticks (68), feeding adult female ticks (95) and male adult ticks (94 clones). Unattached ‘frustrated’ ticks were held in fabric bags affixed to cattle for up to 24 h to identify genes up-regulated prior to host penetration. Sequence analysis was based on BLAST, Panther, KOG and domain (CDD) analyses to assign functional groups for proteins including: cuticle proteins, enzymes (ATPases), ligand binding (histamine binding), molecular chaperone (prefoldin), nucleic acid binding (ribosomal proteins), putative salivary proteins, serine proteases, stress response (heat shock, glycine rich) and transporters. An additional 63% of all contigs and singletons were novel R. microplus transcripts or predicted proteins of unknown function. Expression was confirmed using quantitative real time PCR analysis of selected transcripts. This is the first comprehensive analysis of the R. microplus transcriptome from multiple stages of ticks and assists to elucidate the molecular events during tick attachment and development.
Crown Copyright © 2009 Published by Elsevier B.V. All rights reserved.
Keyword Cattle-arthropoda
Rhipicephalus (Boophilus) microplus
Larvae
RNA
Gene expression
Subtractive hybridization
Amblyomma-cajennense acari
Salivary-glands
Ixodes-scapularis
Cattle-tick
Dermacentor-andersoni
RNA interference
Gene-expression
Soft tick
Ixodidae
Female
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 20 September 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Queensland Alliance for Agriculture and Food Innovation
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 14 Mar 2010, 00:00:21 EST