Screening for grain dormancy in segregating generations of dormant x non-dormant crosses in white-grained wheat (Triticum aestivum L.)

Hickey, Lee T., Dieters, Mark J., DeLacy, Ian H., Christopher, Mandy J., Kravchuk, Olena Y. and Banks, Phillip M. (2009) Screening for grain dormancy in segregating generations of dormant x non-dormant crosses in white-grained wheat (Triticum aestivum L.). Euphytica, 172 2: 183-195. doi:10.1007/s10681-009-0028-z


Author Hickey, Lee T.
Dieters, Mark J.
DeLacy, Ian H.
Christopher, Mandy J.
Kravchuk, Olena Y.
Banks, Phillip M.
Title Screening for grain dormancy in segregating generations of dormant x non-dormant crosses in white-grained wheat (Triticum aestivum L.)
Formatted title
Screening for grain dormancy in segregating generations of dormant x non-dormant crosses in white-grained wheat (Triticum aestivum L.)
Journal name Euphytica   Check publisher's open access policy
ISSN 0014-2336
Publication date 2009-09
Year available 2009
Sub-type Article (original research)
DOI 10.1007/s10681-009-0028-z
Volume 172
Issue 2
Start page 183
End page 195
Total pages 13
Editor Richard G. F. Visser
Place of publication Netherlands
Publisher Springer Netherlands
Collection year 2010
Language eng
Subject C1
820507 Wheat
060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
Formatted abstract
Pre-harvest sprouting (PHS) in wheat (Triticum aestivum L.) is a significant problem. Introgression of genes controlling grain dormancy into white-grained bread wheat is one means of improving resistance to PHS. In this study seven dormant (containing the SW95-50213 and AUS1408 sources) × non-dormant crosses were produced to investigate the effectiveness of selection for grain dormancy in early segregating generations. Each generation (F1–F4) was grown in a temperature controlled glasshouse with an extended photoperiod (i.e. continuous light). F2 and F3 generations were subject to selection. Five hundred harvest-ripe grains were tested for germination over a 14 day period, and the 100 most dormant grains were retained and grown-on to produce the next generation within each cross. The response to selection was assessed through analysis of the time to 50% germination (G50) in the F2, F3 and F4 generations. In addition, changes in marker class frequencies for two SSR markers (barc170 and gpw2279) flanking a known quantitative trait locus (QTL) for grain dormancy on chromosome 4A were assessed in DNA from F2 plants selected from early germinating (non-dormant) and late germinating (dormant) phenotypic extremes within each cross. Selection for grain dormancy in the F2 and F3 generations effectively recovered the dormant phenotype in all seven crosses, i.e. the F4 generation was not significantly different from the dormant parent. Further, selection based on individual F2 grains changed marker class frequencies for the 4A dormancy QTL; in most cases eliminating the marker class homozygous for the non-dormant alleles. Application of this screening method will enable breeders to better select for grain dormancy and may lead to development of new cultivars offering effective resistance to PHS in the near future.
Keyword Pre-harvest sprouting
Phenotypic selection
Single grain germination
Quantitative trait locus
Marker assisted selection
Transgressive segregation
Preharvest Sprouting Tolerance
Seed Dormancy
Qtl
Resistance
Markers
Genes
DNA
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Agriculture and Food Sciences
 
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Created: Sun, 07 Mar 2010, 00:05:40 EST