Clustering of NF-KappaB-related proteins in central and peripheral nodes of Ranvier

Politi, C., Tegeder, I., Del Turco, D., Ghebremedhin, E., Deller, T. and Schultz, C. (2007). Clustering of NF-KappaB-related proteins in central and peripheral nodes of Ranvier. In: The 37th Annual Meeting of Neuroscience, San Diego, California, USA, (). 3-7 November 2007.


Author Politi, C.
Tegeder, I.
Del Turco, D.
Ghebremedhin, E.
Deller, T.
Schultz, C.
Title of paper Clustering of NF-KappaB-related proteins in central and peripheral nodes of Ranvier
Conference name The 37th Annual Meeting of Neuroscience
Conference location San Diego, California, USA
Conference dates 3-7 November 2007
Place of Publication San Diego, CA, United States
Publisher Society for Neuroscience
Publication Year 2007
Sub-type Poster
Language eng
Abstract/Summary Nuclear factor-kappaB (NF-kB) is an ubiquitously expressed transcription factor that modulates inducible gene expression crucial for the regulation of immunity, inflammatory processes, and cell survival. The classical NF-kB heterodimer p50/p65 is mainly controlled by the inhibitory protein IkBa. Phosphorylation of IkBa at Serine 32/36, mediated by the activated IKappaB Kinase (IKK), leads to NF-kB activation. In the nervous system, constitutive NF-kB activation is considered to promote neuronal cell survival by protecting neurons against apoptosis. Recently, we demonstrated a striking enrichment of phosphorylated IkBa (pIkBa) and activated IKK in the axon initial segment (AIS) of neurons (Schultz et al., Mol. Cell. Neurosci. (2006) 33:68-80). Since the AIS shares fundamental structural and functional characteristics with nodes of Ranvier (NR), we examined whether enrichment of pIkBa and activated IKK also occurs in NR. Double immunofluorescent labeling of sections from rodent brain, dorsal root ganglion (DRG), and sciatic nerve was carried out using antibodies against pIkBa, activated IKK, and bIV-spectrin, an established marker for AIS and NR. Interestingly, both pIkBa and activated IKK accumulated in central and peripheral NR. Immunolabeling for these two proteins extended from the nodal domain into the adjacent paranode. Laser scanning microscopy demonstrated that pIkBa predominantly accumulated within the cytoplasm and was associated with fasciculated microtubules. This association was confirmed by pre-embedding immunoelectron microscopy. By comparison, activated IKK preferentially clustered beneath the cytoplasmic membrane. The coincident accumulation of pIkBa and activated IKK in AIS and NR suggests that these specific axonal compartments contribute to neuronal NF-kB regulation.
Subjects 1109 Neurosciences
1103 Clinical Sciences
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Poster number: 238.6/D15 Session: Axon Growth and Guidance: Regulation of the Cytoskeleton

 
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Created: Wed, 03 Mar 2010, 14:02:15 EST by Laura McTaggart on behalf of Faculty of Science