A novel duplex real-time reverse transcription PCR Assay for the detection of influenza A and the novel influenza A(H1N1)strain.

Rockett, Rebecca J., Bialasiewicz, Seweryn, Whiley, David M., Bletchly, Cheryl, Faux, Cassandra E., Lambert, Stephen B., Nimmo, Graeme R., Nissen, Michael D. and Sloots, Theodorus P. (2009) A novel duplex real-time reverse transcription PCR Assay for the detection of influenza A and the novel influenza A(H1N1)strain.. Viruses, 1 3: 1204-1208.


Author Rockett, Rebecca J.
Bialasiewicz, Seweryn
Whiley, David M.
Bletchly, Cheryl
Faux, Cassandra E.
Lambert, Stephen B.
Nimmo, Graeme R.
Nissen, Michael D.
Sloots, Theodorus P.
Title A novel duplex real-time reverse transcription PCR Assay for the detection of influenza A and the novel influenza A(H1N1)strain.
Journal name Viruses   Check publisher's open access policy
Publication date 2009-12-09
Sub-type Article
Year available 2009
DOI 10.3390/v1031204
Volume number 1
Issue number 3
ISSN 1999-4915
Start page 1204
End page 1208
Total pages 5
Editor Dr Eric D Freed
Place of publication Basel, Switzerland
Publisher Molecular Diversity Preservation International (M D P I)
Collection year 2010
Language eng
Subject 060502 Infectious Agents
060506 Virology
C1
920203 Diagnostic Methods
920109 Infectious Diseases
920115 Respiratory System and Diseases (incl. Asthma)
Abstract Abstract: Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(HINl) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU- TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel HINI 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex R T - PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel HINI pandemic strain, in clinical samples.
Keyword influenza
H1N1
real-time
PCR
detection
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Wed, 03 Mar 2010, 13:45:20 EST by Lesley Arnicar on behalf of Clinical Medical Virology Centre